Efficient vitrification freezing method for bovine in-vitro embryo production

A vitrification, bovine body technology, applied in biochemical equipment and methods, cell dissociation methods, preservation of human or animal bodies, etc., can solve the problems of limited application, low pregnancy rate of transplantation, etc. The effect of efficiency
CN114540283APending Publication Date: 2022-05-27INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2022-05-27

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Abstract

The invention provides an efficient vitrification freezing method for bovine in-vitro embryo production. According to the method, beta-nicotinamide mononucleotide is applied to a bovine IVF embryo vitrification technology in bovine in-vitro embryo production for the first time, the beta-nicotinamide mononucleotide with a certain concentration is added into a bovine oocyte in-vitro maturation solution and a bovine in-vitro fertilization embryo culture solution, and then the bovine in-vitro embryo is subjected to vitrification. The vitrification efficiency of the bovine IVF embryo can be greatly improved. The vitrification efficiency can be further improved by further adding growth factors and gap-linked proteins into a bovine oocyte in-vitro maturation solution and a bovine in-vitro fertilized embryo culture solution according to a proportion. Furthermore, by adding the nanoparticles with a certain concentration into the vitrification freezing liquid, the vitrification freezing efficiency can be further improved.
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Description

technical field

[0001] The invention relates to the technical field of bovine in vitro embryo production, in particular to a high-efficiency vitrification and freezing method for bovine in vitro embryo production. Background technique

[0002] The production of bovine in vitro embryos has important economic and social value. It can be used to provide embryo sources for cloning, nuclear transfer, and transgenic animal production, rapidly multiply excellent livestock groups, and protect endangered animals as a means of germplasm resource preservation. It is of great importance theoretical and social significance. However, the post-frozen transfer pregnancy rate of bovine in vitro embryo production (35.89+3.87%) was significantly lower than that of fresh in vitro embryo production (51.35+1.87%), which greatly limited their application (Niu et al., 2014). According to IETS statistics, from 1997 to 2017, the global bovine in vitro embryo transfer was dominated by fresh embryo tr...

Claims

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