CD4 + T lymphocyte automatic counting detection system based on micro-fluidic chip

Abstract

The invention relates to the technical field of life medicine detection, in particular to an automatic CD4 + T lymphocyte counting and detecting system based on a micro-fluidic chip, which is simple and convenient to operate, low in cost and miniaturized, and comprises the micro-fluidic chip, the micro-fluidic chip comprises a structural sheet, a reaction cavity and a channel located at the lower part of the reaction cavity, the reaction cavity and the channel are both located in the structural sheet, and the structural sheet is provided with a cavity. The movement mechanism comprises a linear motor, a sliding block and a detection paper strip, the detection paper strip comprises a sample absorption area and a paper strip testing area, the detection device comprises a photoelectric detector used for scanning the paper strip testing area, an electromagnet is arranged on the side face of the channel, a baffle hinged to the inner wall of the channel is arranged on one side of the bottom of the channel, and a check block is arranged on the other side of the bottom of the channel. The baffle can be buckled with the check block to seal the channel, a magnetic block is arranged in the baffle, and the magnetic block can be attracted by the electromagnet so that the baffle can be separated from the check block to open the channel. The full-process automatic operation of capturing, separating and detecting the CD4 + T lymphocytes can be realized.

Description

technical field [0001] The invention relates to the technical field of life medicine detection, in particular to a CD4+T lymphocyte automatic counting and detection system based on a microfluidic chip. Background technique [0002] CD4+T lymphocytes are an important immune cell in the human immune system. Since the target of HIV attack is CD4+T lymphocytes, the test results provide the clearest indicator of the damage of the immune system of HIV-infected patients. Detection of CD4+T lymphocytes Lymphocytes are of great significance: (1) Monitoring of disease progression: CD4+ T lymphocyte detection is generally performed every 6-12 months, and the patient's disease progression is judged by the count of CD4+ T lymphocytes. (2) Risk assessment of opportunistic infections: Opportunistic infections are the main cause of death in AIDS patients, and CD4+ T lymphocytes can assess the risk of opportunistic infections in HIV-infected patients and assist in judging whether to take pre...

AI Technical Summary

Problems solved by technology

[0004] In the existing detection methods, it is usually necessary to rely on a large-scale flow cytometer for the detection of CD4+ T lymphocytes. This instrument is expensive, the detection process is complicated, and the detection cost is high. Therefore, it is necessary to develop an easy-to-operate, low-cost, Miniaturized and automated automatic counting and detection system for CD4+ T lymphocytes

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