Culture medium for efficiently inducing wheat anther callus
A culture medium, wheat technology, applied in cell culture medium, plant cells, etc., can solve the problem of low recovery rate of microspores, and achieve the effects of improving the recovery rate, promoting growth, and early recovery time.
Pending Publication Date: 2022-06-03
河北省农林科学院生物技术与食品科学研究所
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Problems solved by technology
[0010] The present invention proposes a medium for efficiently inducing wheat anthers to heal, which solves the problem of low microspore healing rate in flower cultivation species in the prior art
Method used
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Embodiment 1
[0067] A medium for efficiently inducing the emergence of wheat anthers. On the basis of the existing W14 medium, the carbon source is adjusted, that is, sucrose is adjusted to glucose, and the mass concentration of the glucose is 54000 mg / L.
Embodiment 2
[0069] A medium for efficiently inducing the emergence of wheat anthers. On the basis of the existing W14 medium, the nitrogen source is adjusted, that is, the inorganic reduced nitrogen is adjusted to the organic reduced nitrogen, and the organic reduced nitrogen includes L-glutamine, The mass concentration mg / L of each component of L-arginine is: L-glutamine 1000, L-arginine 170.
Embodiment 3
[0071] A medium for efficiently inducing the emergence of wheat anthers. On the basis of the existing W14 medium, the nitrogen source is adjusted, that is, the inorganic reduced nitrogen is adjusted to the organic reduced nitrogen, and the organic reduced nitrogen includes L-glutamine, L-arginine, L-aspartine, glycine, the mass concentration mg / L of each component is: L-glutamine 1000, L-arginine 170, L-aspartin 130, glycine 50.
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Abstract
The invention relates to the technical field of anther breeding, and provides a culture medium for efficiently inducing wheat anther callus, which comprises the following steps: adjusting a nitrogen source: removing inorganic reducing nitrogen, and adjusting the reducing nitrogen into organic reducing nitrogen; the organic reduced nitrogen comprises L-glutamine and L-arginine, or the organic reduced nitrogen comprises L-glutamine, L-arginine, L-asparagine and glycine, or the organic reduced nitrogen comprises L-glutamine, L-arginine, L-asparagine and glycine; and / or adjusting the carbon source: adjusting the cane sugar into glucose. By adjusting the nitrogen source and / or the carbon source, the callus rate of the wheat anther is obviously improved. According to the PCI culture medium designed by the invention, wheat varieties' Jinghuang 9123 'and' Kenong 199 'are taken as test materials, and compared with the best wheat anther culture medium which is currently considered by people, the callus rate can be increased by at least 20%, the callus time is about one week earlier, the growth speed of calluses is high, and the survival rate of the calluses is high. The method has the advantages that more gene recombinants can be captured during breeding application, and the haploid obtaining efficiency is improved.
Description
technical field [0001] The invention relates to the technical field of flower cultivation, in particular to a medium for efficiently inducing wheat anthers to emerge. Background technique [0002] The anther of a plant is cultivated with a medium, the stemness of the haploid microspores is mobilized, and it is induced into a callus that can divide continuously and has the ability to regenerate a complete plant (that is, totipotency), which is a plant flower cultivation. Important technical key. The purpose of this is that by doubling the haploid, the lengthy process of self-purification of the hybrid progeny can be omitted, thereby greatly accelerating the breeding process. Because wheat has a complex and huge genome, it is objectively determined that the wheat flower cultivar will have more chances to retain or select excellent genetic variation and the necessity of accelerating purification by haploid doubling. [0003] A lot of research and application practice show tha...
Claims
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IPC IPC(8): C12N5/04
CPCC12N5/0025
Inventor 王海波张俊敏周硕吕孟雨董福双刘永伟赵和柴建方张洪伟马秀英李雯马琳峰吕爱枝
Owner 河北省农林科学院生物技术与食品科学研究所