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Novel collagen and bioactive ceramic composite bone grafting material and preparation method thereof

A technology of bioactive ceramics and bone grafting, applied in tissue regeneration, prosthesis, medical science, etc., can solve the problems of excessive inflammatory response of the immune system, affecting the integration of implants and bone tissue, affecting rehabilitation, etc., to promote proliferation and differentiation , to achieve the effect of immune regulation and convenient operation

Active Publication Date: 2022-06-07
BEIJING NATON INST OF MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process is mainly due to the excessive inflammatory response of the immune system caused by the invasion of foreign bacteria, which promotes the formation of fibrous connective tissue on the surface of the bone graft material, which seriously affects the combination between the implant and bone tissue, thereby affecting postoperative recovery.

Method used

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  • Novel collagen and bioactive ceramic composite bone grafting material and preparation method thereof
  • Novel collagen and bioactive ceramic composite bone grafting material and preparation method thereof
  • Novel collagen and bioactive ceramic composite bone grafting material and preparation method thereof

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preparation example Construction

[0074] Another aspect of the embodiment of the present invention also provides a preparation method of the composite bone graft material, comprising the following steps:

[0075] (1) adding phosphatidylserine to the collagen slurry, mixing uniformly to prepare collagen / phosphatidylserine slurry;

[0076] (2) Mixing the bioactive ceramics and the collagen / phosphatidylserine slurry uniformly, freezing into a block, and then freeze-drying to obtain a collagen / bioactive ceramic / phosphatidylserine scaffold material;

[0077] (3) The chitosan quaternary ammonium salt is grafted onto the collagen / bioactive ceramic / phosphatidylserine scaffold material through the inoculation process, washed, and freeze-dried to prepare the composite bone graft material.

[0078] The preparation method of the composite bone graft material according to the embodiment of the present invention is relatively simple, easy to operate, and low in cost, and can be widely used in the field of bone tissue engine...

Embodiment 1

[0094] Example 1: (Collagen / Bioactive Glass / Phosphatidylserine / Hydroxypropyltrimethylammonium Chloride Chitosan)

[0095] A preparation method of collagen / bioactive glass / phosphatidylserine / hydroxypropyltrimethylammonium chloride chitosan composite bone graft material, comprising the following steps:

[0096] (1) Collagen dissolution: Dissolve 6g type I collagen sponge in 113ml water for injection, stir for 3h, put it in a 4°C refrigerator and let stand for 24h to form a uniform collagen slurry;

[0097] (2) Collagen / phosphatidylserine mixing: take 6g of phosphatidylserine and mix it into the prepared collagen slurry, stir at 4°C for 5 minutes, and mix evenly to obtain collagen / phosphatidylserine slurry, which is ready for use;

[0098] (3) Collagen / phosphatidylserine / bioactive glass blend: take 75g Na 2 O-CaO-SiO 2 -P 2 O 5 It is a bioactive glass, and then the bioactive glass is mixed into the collagen / phosphatidylserine slurry, stirred at 4°C for 5 minutes, and the coll...

Embodiment 2

[0104] The results of cytotoxicity showed that the cell survival rate was 88.66% after co-culturing the material system with L929 cells for 24 hours; the cell proliferation results showed that the OD value of the material system was 0.81±0.01 after co-culturing with MC3T3-E1 cells for 72 hours; the antibacterial experiment The results showed that the OD value of the material system was 0.13 ± 0.02 after co-cultivation with Staphylococcus aureus for 48 hours; the osteogenesis experiment showed that the ALP activity of the material system and BSMCs cells was 5.08 ± 0.06 mg / ml after co-cultivation with BSMCs cells for 14 days; The results of cell phenotype analysis showed that the content of IL-10 secreted by macrophages after co-culture with RAW264.7 cells for 72 h was 7.76 pg / ml, and the content of secreted TNF-α was 320.06 pg / ml; The mass loss of the material system was 42.62% after soaking it for a day; comparing the above results with collagen / bioglass / phospholipid serine (Co...

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Abstract

The invention discloses a novel collagen and bioactive ceramic composite bone grafting material and a preparation method thereof, and belongs to the field of biomedical materials.The composite bone grafting material comprises collagen, bioactive ceramic, chitosan quaternary ammonium salt and phosphatidylserine, according to the composite bone grafting material, phosphatidylserine-doped collagen and bioactive ceramic are used as scaffold materials, and chitosan quaternary ammonium salt is grafted on the surface of the scaffold materials. The composite bone grafting material disclosed by the invention can realize gradient regulation and control of sterilization, inflammatory response and osteoblast proliferation and differentiation, and can effectively inhibit operation infection, reduce inflammatory response, improve bone induction capability and realize efficient bone tissue repair.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a novel collagen and bioactive ceramic composite bone graft material and a preparation method thereof. The composite bone graft material has immunoregulatory functions, osteopromoting properties and antibacterial properties, and can meet clinical requirements for high-performance materials. requirements. Background technique [0002] After the bone tissue is damaged, bone graft repair is generally required, and postoperative infection is an important reason for the failure of the operation. This process is mainly due to the excessive inflammatory response of the immune system caused by the invasion of foreign bacteria, which promotes the formation of fibrous connective tissue on the surface of the bone graft material, which seriously affects the combination between the implant and the bone tissue, thereby affecting postoperative rehabilitation. [0003] Inflammatory reaction is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/10A61L27/12A61L27/20A61L27/36A61L27/50A61L27/54A61L27/58
CPCA61L27/24A61L27/10A61L27/12A61L27/20A61L27/3608A61L27/365A61L27/50A61L27/54A61L27/58A61L2430/02A61L2430/12A61L2300/412A61L2300/41A61L2300/602A61L2300/404A61L2300/236A61L2300/216C08L5/08
Inventor 李祥瑞李梅董骧
Owner BEIJING NATON INST OF MEDICAL TECH CO LTD
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