Delivery system based on nano-hydroxyapatite, preparation method, application, pharmaceutical composition, spray and hydrogel

A nano-hydroxyapatite and delivery system technology, which is applied in the direction of drug combination, active ingredients of phosphorus compounds, drug delivery, etc., can solve problems such as insignificant dental caries effect, weakened drug effect, and bacterial resistance to drugs. Effects of inhibiting re-aggregation, improving binding affinity, and preventing further deterioration

Active Publication Date: 2022-06-24
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, long-term use of antibiotics is likely to cause bacterial resistance to the drug, thereby weakening the effect of the drug
[0005] Although the above methods can prevent the occurrence of caries, because oral pathogenic bacteria will produce a large amount of

Method used

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  • Delivery system based on nano-hydroxyapatite, preparation method, application, pharmaceutical composition, spray and hydrogel
  • Delivery system based on nano-hydroxyapatite, preparation method, application, pharmaceutical composition, spray and hydrogel
  • Delivery system based on nano-hydroxyapatite, preparation method, application, pharmaceutical composition, spray and hydrogel

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preparation example Construction

[0051] In another exemplary embodiment of the present invention, a preparation method of the aforementioned nano-hydroxyapatite-based delivery system is provided, which specifically includes the following steps:

[0052] S1, adding nano-hydroxyapatite to the aqueous solution of the chitosan derivative, and performing ultrasonication, centrifugation and filtration to obtain a first suspension.

[0053] S2, dissolving the photosensitizer in dimethyl sulfoxide to obtain a photosensitizer solution, adding the photosensitizer solution to the first suspension to obtain a second suspension, and removing free substances in the second suspension, The delivery system is obtained.

[0054] In a preferred embodiment, the specific process of the step S1 is as follows:

[0055] The chitosan derivative is added to deionized water to obtain an aqueous solution of the chitosan derivative.

[0056] Nano-hydroxyapatite is added to the aqueous solution of the chitosan derivative, sonicated in a...

Embodiment 1

[0070] 40 mg of QCS was added to 20 mL of deionized water to obtain an aqueous QCS solution.

[0071] 20 mg of nHAP was added to the QCS aqueous solution, in a cell sonicator, at a temperature of 4 °C, with an ultrasonic power of 150 W, sonicated for 1 h (2 s per sonication, with an interval of 4 s), and then the supernatant was collected by high-speed centrifugation.

[0072] The supernatant was filtered through a microporous membrane to remove unbound material to obtain a QCS / nHAP nanosuspension.

[0073] Weigh 10 mg Ce6 and dissolve it in 10 mL DMSO to prepare a 1 mg / mL Ce6 solution, which is refrigerated for later use. 200 μL Ce6 was added to 1 mL QCS / nHAP nanoparticle suspension.

[0074] Use Sephadex column G-25 to remove small molecules to obtain Ce6@QCS / nHAP.

Embodiment 2

[0076] 20 mg of N,N-trimethyl chitosan quaternary ammonium salt was added to 20 mL of deionized water to obtain an aqueous solution of N,N-trimethyl chitosan quaternary ammonium salt.

[0077] Add 20 mg of nHAP to the N,N-trimethyl chitosan quaternary ammonium salt aqueous solution, in a cell sonicator, at a temperature of 10 °C, with an ultrasonic power of 100W, ultrasonication for 0.5h (2s per ultrasonic, 4s interval). ), followed by high-speed centrifugation to remove the supernatant.

[0078] The supernatant was filtered through a microporous membrane to remove unbound material to obtain N,N-trimethyl chitosan quaternary ammonium salt / nHAP nanosuspension.

[0079] 5 mg of porphine was weighed and dissolved in 10 mL of DMSO to obtain a 0.5 mg / mL Ce6 solution, which was refrigerated for later use. 80 μL of porphine was added to 1 mL of N,N-trimethyl chitosan quaternary ammonium salt / nHAP nanosuspension.

[0080] Use Sephadex column G-25 to remove small molecules to obtain ...

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Abstract

The invention provides a delivery system based on nano-hydroxyapatite, the delivery system comprises nano-hydroxyapatite, the surface of the nano-hydroxyapatite is coated with a chitosan derivative to obtain a first nano-system, and the surface of the first nano-system is loaded with a photosensitizer; wherein the chitosan derivative is a water-soluble chitosan derivative. The invention also provides a preparation method and application of the delivery system based on nano-hydroxyapatite, a pharmaceutical composition, a spray and hydrogel. The delivery system provided by the invention can prevent further deterioration of decayed teeth, prevent demineralization of the teeth, maintain the microenvironment on the surfaces of the teeth, and realize a remineralization effect on damaged enamel.

Description

technical field [0001] The present invention relates to the technical field of nano-photosensitive materials, in particular to a delivery system, preparation method, application, pharmaceutical composition, spray and hydrogel based on nano-hydroxyapatite. Background technique [0002] The oral cavity is one of the important microbial reservoirs in the human body, with more than 700 species of bacteria. The oral cavity provides a good growth and reproduction environment for microorganisms, whereby a variety of microorganisms colonize the surfaces of teeth, prostheses and mucosa to form a biological community. Dental caries is a bacterial infection caused by the carious biofilm formed on the tooth surface to accelerate the process of tooth demineralization. It is one of the most common oral diseases. [0003] During the development of dental caries, the tooth surface exposed to the oral environment, due to long-term exposure to the oral environment containing fermentable carb...

Claims

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Application Information

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IPC IPC(8): A61K9/12A61K9/06A61K47/02A61K47/36A61K41/00A61K33/42A61P1/02A61P31/02
CPCA61K9/006A61K9/0063A61K9/06A61K47/02A61K47/36A61K41/0057A61K41/0071A61K33/42A61P1/02A61P31/02A61K2300/00Y02A50/30
Inventor 缪文俊李媛媛郭文靓
Owner NANJING UNIV OF TECH
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