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Application of CXCL10+CD274+CD68 + cell population in preparation of chronic hepatitis B prognosis prediction product

A chronic hepatitis B, CD274 technology, applied in the field of biomedicine, can solve the problems of inability to accurately locate, hinder the development of new treatment methods, and lack of early prognosis biomarkers.

Pending Publication Date: 2022-06-24
THE THIRD PEOPLES HOSPITAL OF SHENZHEN
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AI Technical Summary

Problems solved by technology

In the field of chronic hepatitis B (CHB) research, there is still a lack of understanding of the immunosuppressive mechanism in the liver microenvironment of CHB patients, the diversity of immune cells and stromal cells, the interaction mechanism of immune cells and stromal cells, and the induction of disease. A comprehensive understanding of the cell types and molecular pathways through which development occurs, leading to hampering the development of novel therapeutics
The main shortcomings of the current research: (1) The limitations of limited experimental methods. At present, flow cytometry and other methods are mostly used for research, and only a small number of biomarkers can be used to search for immune cell changes in the liver of chronic hepatitis B at a coarse granularity. , Unable to pinpoint immune cell subsets and biomarkers critical to viral control and disease progression in chronic hepatitis B
(2) There are individual differences in chronic hepatitis B virus control and disease prognosis, lack of early prognosis biomarkers, and early warning and intervention for patients with poor prognosis

Method used

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  • Application of CXCL10+CD274+CD68 + cell population in preparation of chronic hepatitis B prognosis prediction product
  • Application of CXCL10+CD274+CD68 + cell population in preparation of chronic hepatitis B prognosis prediction product
  • Application of CXCL10+CD274+CD68 + cell population in preparation of chronic hepatitis B prognosis prediction product

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Experimental program
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Embodiment 1

[0028] according to figure 1 The process of 6 healthy people and 5 people with low hepatitis B viral load (average viral load was 5 × 10 4 U / ml) chronic hepatitis B patients, 9 patients with high hepatitis B viral load (average viral load was 2 × 10 8 U / ml) liver biopsy specimens of patients with chronic hepatitis B were used as materials for experiments, and the specific steps were as follows:

[0029] Grinding, tissue digestion, and sequencing of single-cell libraries

[0030] Liver biopsy specimens were first cut into small pieces (about 0.5 mm each) with scissors. 2 ) were homogenized, and then liver tissue was digested with 0.3 mg / ml of collagenase type IV (Sigma, C9891) and DNAse I (Sigma, D5025) for 1 hour at 37°C. Digestion was then interrupted by the addition of 5 ml of Dulbecco's Phosphate Buffered Saline (DPBS, Thermo, 14190250) and 5% FBS, followed by centrifugation of the dissociated supernatant for 5 minutes. Red blood cells were lysed using ammonium chloride...

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Abstract

The invention discloses application of a substance for detecting the proportion of a CXCL10 < + > CD274 < + > CD68 < + > cell population in all CD68 < + > immune cells in a sample in preparation of a product for predicting prognosis of a chronic hepatitis B patient. The result of one embodiment of the invention shows that the proportion of CXCL10 < + > CD274 < + > CD68 < + > cell populations in human liver cells in all CD68 < + > immune cells is highly related to virus control and prognosis of chronic hepatitis B.

Description

technical field [0001] The invention relates to the application of CXCL10+CD274+CD68+ cell group in the preparation of chronic hepatitis B prognosis prediction products in the field of biomedicine. Background technique [0002] The natural history of HBV infection is complex and variable, influenced by complex interactions between the host immune system and the virus. In the field of chronic hepatitis B (CHB) research, there is still a lack of understanding of the immunosuppressive mechanism in the liver microenvironment of CHB patients, the diversity of immune cells and stromal cells, the interaction mechanism of immune cells and stromal cells, and the induced disease. A comprehensive understanding of the cell types and molecular pathways in which development occurs, thereby hindering the development of novel therapeutic approaches. The main shortcomings of the current research: (1) The limitations of limited experimental methods, etc. Currently, flow cytometry and other m...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/576G01N15/14
CPCG01N33/6872G01N33/6863G01N33/5761G01N33/56966G01N15/14G01N2015/1486G01N2800/52
Inventor 刘阳许刚赵娟娟张政杨倩婷汪海燕
Owner THE THIRD PEOPLES HOSPITAL OF SHENZHEN
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