Construction method of zebra fish obesity model

A technology of obesity model and construction method, applied in the field of construction of zebrafish obesity model, can solve the problems of strong operability and low mortality, and achieve the effects of good economy, reduced mortality and high efficiency

Pending Publication Date: 2022-06-28
ZHEJIANG RES INST OF CHEM IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention uses two kinds of chemical substances and dietary induction in combination, and has good effect, low mortality rate and strong operability, and there is no reported application yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] AB strain zebrafish 10 male zebrafish and 5 female zebrafish were placed in the mating box, and the eggs were collected after mating. Healthy eggs are incubated at a constant pH of 6.5-7.5, dissolved oxygen 10.5±0.5mg / L, hardness 200-250mg / L (with CaCO 3 meter), incubate in tap water dechlorinated and aerated by activated carbon at a water temperature of 27±1°C. On the 7th day after fertilization, 200 healthy zebrafish larvae were selected and randomly divided into 2 groups, which were fed in glass tanks (10L), one group was the treatment group and the other was the solvent control group. In the treatment group, BPA and DEHP were both assisted by acetone to prepare solutions with a concentration of 25 μg / L and 50 μg / L, respectively, and the volume fraction of acetone in each solution was not greater than 1×10 -5 , and then the two solutions were uniformly mixed in equal volumes, continuously stirred and aerated for 24 hours to obtain a mixed aqueous solution, which was...

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PUM

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Abstract

The invention discloses a method for constructing a zebra fish obesity model, which comprises the following steps of: placing male and female adult zebra fish in a mating box, collecting eggs after mating, culturing and hatching healthy eggs at constant temperature, and selecting healthy young zebra fish after fertilization; bPA and DEHP are dissolved with acetone to prepare a mixed aqueous solution, and the mixed aqueous solution is used for feeding the juvenile zebrafish; and after hatching, feeding cooked egg yolk until the 21st day after fertilization, and building a zebra fish obesity model. The method for constructing the zebra obesity model has the advantages of being short in time, good in economical efficiency, high in efficiency and the like, and a convenient, rapid and efficient research tool can be provided for toxicity assessment and mechanism research of environmental endocrine disrupters, treatment drug screening and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for constructing a zebrafish obesity model. Background technique [0002] Obesity is a chronic metabolic disease caused by the interaction of genetic factors, environmental factors and other factors. Its mechanism is that energy intake exceeds consumption, resulting in excessive accumulation of body fat and excess body weight by more than 20%. At present, obesity has become one of the most common chronic diseases in the world. [0003] Animal models are an indispensable means and method to study obesity, and have received more and more extensive attention. The use of zebrafish to replace the traditional model of rats and mice is in line with the principles of animal protection, and has many advantages such as reducing modeling costs, shortening modeling cycles, and being sensitive to drug reactions. It has always been the object of academic research. [0004] The p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K10/20A23K20/111A23K20/105A23K50/80
CPCA01K61/10A23K10/20A23K20/111A23K20/105A23K50/80Y02A40/81
Inventor 岑江杰李楠崔彦丽郑欢图张武纪磊钱浙斌黄慧谭头云方华
Owner ZHEJIANG RES INST OF CHEM IND CO LTD
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