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Tissue culture and rapid propagation improvement method and device for sunshine rose grapes

A technology of tissue culture rapid propagation and sunshine rose, applied in the biological field, can solve the problems of low efficiency and long time, and achieve the effect of avoiding time and energy, and solving the problem of long time and low efficiency.

Pending Publication Date: 2022-07-01
桂林市农业科学研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide an improved method and device for tissue culture and rapid propagation of Sunshine Rose grape, aiming to solve the problem of time-consuming and low efficiency of the existing technology of Sunshine Rose grape tissue culture and rapid propagation

Method used

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  • Tissue culture and rapid propagation improvement method and device for sunshine rose grapes
  • Tissue culture and rapid propagation improvement method and device for sunshine rose grapes
  • Tissue culture and rapid propagation improvement method and device for sunshine rose grapes

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Embodiment Construction

[0025] The following describes in detail the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary, and are intended to explain the present invention and should not be construed as limiting the present invention.

[0026] In the description of the present invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientations or positional relationships indicated by "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientations or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, rather...

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Abstract

The invention relates to the technical field of biology, in particular to a sunshine rose grape tissue culture and rapid propagation improvement method and device. The explants are disinfected; carrying out primary culture on the disinfected explants to obtain robust bud seedlings; carrying out rooting culture on the robust bud seedlings obtained by primary culture to obtain rooted seedlings; and hardening the rooted seedlings, and putting the seedlings into the ground. The ratio of a primary culture medium to a rooting culture medium is improved, the primary germination rate and the rooting rate are greatly increased, conventional subculture is not carried out, complex procedures and uncontrollable pollution factors of subculture are avoided, and under the condition that production conditions and manual operation are basically consistent, the production efficiency is greatly improved. The conventional tissue culture needs 3 months for producing a batch of healthy nursery stocks (based on 1000 plants), and only 45 days are needed after the method is improved, so that the problems of long time consumption and low efficiency of the existing sunshine rose grape tissue culture and rapid propagation technology are solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a device for improving tissue culture and rapid propagation of Sun rose grapes. Background technique [0002] The application of tissue culture rapid propagation technology is of great significance in the rapid propagation of new varieties into seedlings, cost reduction, and variety improvement. In addition, the cultivation of virus-free seedlings is also mostly based on tissue culture technology. [0003] The purpose of conventional subculture in tissue culture is to expand the plant multiples, but the value-added coefficient of Sunshine Rose grapes is small, generally around 1-2, and the subculture requires at least 30 days of time and repeated transfer, which is a waste of time. Time and increased workload with little effect. SUMMARY OF THE INVENTION [0004] The purpose of the present invention is to provide an improved method and device for tissue culture and rap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G7/04
CPCA01H4/008A01H4/002A01H4/001A01G7/045
Inventor 张丹钟坤李家文
Owner 桂林市农业科学研究中心
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