Preparation method and application of stem cell exosome concentrated solution gel preparation

A technology of gel preparations and exosomes, applied in the field of gel preparations, can solve problems such as complicated methods, high treatment risks, and limited treatment effects, and achieve the effects of increasing stability and activity, promoting angiogenesis, and improving treatment effects

Pending Publication Date: 2022-07-01
四川中天鑫源生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a stem cell exosome concentrate gel formulation for the current erectile dysfunction mainly treated by intravenous injection, the treatment effect is limited and the method of direct injection into the penis is relatively complicated and the treatment risk is high. Preparation method and its application

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  • Preparation method and application of stem cell exosome concentrated solution gel preparation
  • Preparation method and application of stem cell exosome concentrated solution gel preparation
  • Preparation method and application of stem cell exosome concentrated solution gel preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0033] This embodiment provides a preparation method of a stem cell exosome concentrate gel preparation, comprising the following steps:

[0034] S1 selects human umbilical cord mesenchymal stem cells and provides medicinal polymer stabilizer (sodium hyaluronate), moisturizing agent (propylene glycol and glycerol), transdermal absorption enhancer (dimethyl sulfoxide), and compound electrolyte injection.

[0035] The selection criteria of the human umbilical cord mesenchymal stem cells are as follows: from the human umbilical cord mesenchymal stem cells of the P2-P8 generation, the stem cells with good growth status and negative microbial detection are selected. The compound electrolyte solution is produced by Sichuan Kelun Pharmaceutical Co., Ltd. The compound electrolyte solution contains 5.26g of sodium chloride, 5.02g of sodium gluconate, 3.68g of sodium acetate, 0.37g of potassium chloride and 0.30g of magnesium chloride per 1000ml of the compound electrolyte solution. . ...

Embodiment 2

[0051] This embodiment provides a stem cell exosome concentrate gel preparation, which is prepared by the preparation method of a stem cell exosome concentrate gel preparation as in Example 1. The components of each 100ml of the stem cell exosome concentrate gel preparation are as follows: umbilical cord mesenchymal stem cell exosome concentrate 50ml, humectant (propylene glycol and glycerol) 8ml, transdermal absorption enhancer (dimethyl sulfoxide) ) 8ml, medicinal polymer stabilizer (sodium hyaluronate) 10g, and the balance is compound electrolyte injection.

[0052] In this example, the exosome components of the umbilical cord mesenchymal stem cell exosome concentrate have biological activity, and the release of cytokines (such as VEGF and fibroblast growth factor) in the exosomes can promote angiogenesis and can regulate The local tissue microenvironment can improve the local blood supply, so as to achieve the effect of improving the blood supply to the penis and treating ...

experiment example 1

[0057] The patient Zhai Mou (37 years old), a middle-level leader of the unit, due to busy work and high pressure, suffered from loss of libido, decreased frequency of sexual life a year ago, and early symptoms of erectile dysfunction. Before treatment, he had sex within 3 months The average frequency was 1 time per week, and 3 of them did not complete the sex life because the penis could not be erected normally. 4 times during intercourse the penis failed to maintain an erection before ejaculation, and ejaculation failed. The other 6 completed the sexual intercourse, with an average time of less than 3 minutes. According to the "International Erectile Function Index" self-assessment score of 12 points, it belongs to mild erectile dysfunction.

[0058] The patient was treated with the gel preparation of stem cell exosome concentrate (the treatment cycle was four weeks). After washing the penis in the morning, noon and before going to bed at night, the gel preparation of stem ...

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Abstract

The invention discloses a preparation method and application of a stem cell exosome concentrated solution gel preparation. The preparation method comprises the following steps: S1, selecting human umbilical cord mesenchymal stem cells, and providing a medicinal polymer stabilizer, a humectant, a transdermal absorption enhancer and a compound electrolyte injection; s2, performing starvation culture-enrichment concentration on the human umbilical cord mesenchymal stem cells to obtain an umbilical cord mesenchymal stem cell exosome concentrated solution. S3, mixing to obtain the stem cell exosome concentrated solution gel preparation. Each 100ml of the stem cell exosome concentrated solution gel preparation is prepared from the following components: 50ml of umbilical cord mesenchymal stem cell exosome concentrated solution, 8ml of humectant, 8ml of transdermal absorption enhancer, 10g of medicinal polymer stabilizer and the balance of compound electrolyte injection. According to the present invention, the human umbilical cord mesenchymal exosome extract can be obtained within a short time, and the stable and uniform gel-like substance easy to absorb can be prepared so as to provide convenience for the treatment of male erectile dysfunction.

Description

technical field [0001] The present invention relates to a gel preparation, in particular to a gel preparation of a stem cell exosome concentrate, and also to the application of the gel preparation. Background technique [0002] Erectile dysfunction (ED) refers to the disease in which the inability to achieve and maintain an erection and to achieve satisfactory sexual intercourse is the main manifestation. It is the most common male dysfunction disease. The course of the disease lasts for more than 3 months. For organic, surgical, traumatic, mental and penile diseases, the conventional treatment method is oral drug treatment, but this treatment method is highly dependent on drugs and cannot achieve the purpose of radical cure. [0003] In addition, studies have found that umbilical cord mesenchymal stem cells have multiple functions such as multi-directional differentiation, tissue repair, and immune regulation. Because of their extremely low immunogenicity, allogeneic use wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/06A61K47/36A61P15/10C12N5/0775
CPCA61K35/28A61K9/06A61K47/36A61K9/0031A61P15/10C12N5/0668C12N2509/10
Inventor 张倩
Owner 四川中天鑫源生命科技有限公司
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