Maize pollen storage method

Abstract

The invention belongs to the technical field of agricultural breeding production. Aiming at the problems of strict storage conditions and relatively high cost in a corn pollen low-temperature preservation technology, the invention provides a corn pollen storage method, which comprises the following steps of: 1, extracting tassels which just begin to loosen pollen from the top end of a corn plant along with a section of stalk; step 2, putting the taken-down tassel stalks into a container filled with water, binding the tassels by using a preservative film, putting the tassels into a refrigeration house, and preserving the tassels in a dark place; step 3, taking out the tassels from the refrigeration house 6-18 hours ahead of time according to the drawing time of the female tassels, placing the tassels at room temperature, and when pollen is taken, firstly placing a pollen collection container, then unwrapping the preservative film, lightly beating the tassels, and collecting pollen with cracked tassel anther into the container; 4, the collected pollen is sent to the field in time, and corn ear filament pollination is completed. The method is simple to operate and high in pollen extraction efficiency; the storage conditions are simple, and the storage cost is saved.

Description

technical field [0001] The invention belongs to the technical field of agricultural breeding and production, and in particular relates to a method for storing corn pollen. Background technique [0002] Pollen is one of the forms of plant germplasm, which contains all the genotypes of the species, has rich genetic diversity, and is an important material for germplasm conservation and exchange as well as hybrid breeding. However, under natural conditions, the life span of many pollens is very short, which brings great inconvenience to germplasm research and hybrid breeding, and is also not conducive to related plant genetic research. The research on cryopreservation of pollen is of great significance for accelerating the breeding process and improving the conservation methods of germplasm resources. [0003] In order to meet the needs of germplasm resources preservation and exchange and cross-breeding work, research on cryopreservation of pollen is emerging and gradually deep...