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Application and method of isoretinoic acid in preparation of product for controlling transmission of arthroviruses

An arbovirus and isotretinoin technology, applied in the field of biomedicine, can solve the problems that have not been found in the research or report of isotretinoin to reduce or block arboviruses, achieve inhibition or block transmission, reduce contact, and inhibit easy Sensual effect

Pending Publication Date: 2022-07-05
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no related studies or reports that isotretinoin reduces or blocks arboviruses

Method used

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  • Application and method of isoretinoic acid in preparation of product for controlling transmission of arthroviruses
  • Application and method of isoretinoic acid in preparation of product for controlling transmission of arthroviruses
  • Application and method of isoretinoic acid in preparation of product for controlling transmission of arthroviruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0067] The following examples further illustrate the present invention, but are not intended to limit the present invention. Specific materials used in embodiments of the present invention and their sources are provided below. However, it should be understood that these are merely exemplary and are not intended to limit the present invention, and materials with the same or similar types, models, qualities, properties or functions as the following reagents and instruments can be used to implement the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0068] 1. Experimental materials

[0069] Aedes aegypti used in the following examples is in the document "Colpitts™ etal. Alterations in the Aedes aegypti Transcriptome during Infection with WestNile, Dengue and Yellow Fever...

Embodiment 1

[0094] Example 1: Oral administration of isotretinoin to mice infected with dengue virus or Zika virus will significantly reduce the proportion of mosquito bites in mice.

[0095] Mice infected with dengue type 2 virus (DENV2) or Zika virus (ZIKV) were given isotretinoin by gavage at a dose of 1 mg / mice / day in 10% DMSO mixed with corn Oil, administered for three consecutive days after infection. On the third, fourth, and fifth days of dengue virus or Zika virus infection in mice, mosquitoes were allowed to conduct mouse-biting experiments, respectively, and the number of mosquitoes biting normal mice, infected mice, and infected mice was calculated. (number of successful blood-sucking mosquitoes / total number of mosquitoes released). Three mice were used in each group of experiments, and the total number of mosquitoes released by each mouse was 22-36.

[0096] The experimental results see 1A to 1C As well as Table 1 to Table 3 below: On the third, fourth and fifth days of v...

Embodiment 2

[0098] Example 2: Oral administration of isotretinoin to mice infected with dengue virus or Zika virus will significantly reduce the proportion of mosquitoes infected with the virus.

[0099] Eight days after the mosquitoes sucked blood, the total RNA of Aedes aegypti was extracted (RNeasy Mini Kit, 74106, Qiagen), reverse transcribed into cDNA (iScript cDNA synthesis kit, 170-8890, Bio-Rad), and the SYBR qPCR kit ( iTaq universal SYBR Green, 1725120, Bio-Rad) detects the relative expression of dengue type 2 virus genes or Zika virus genes in Aedes aegypti. The relative expression levels of Zika virus genes, using Actin of Aedes aegypti as a gene reference. The ratio of dengue virus / Actin or Zika virus / Actin ≧0.001 was used as the critical value for infection. Dengue virus / Actin or Zika virus / Actin ratio ≧ 0.001 indicates that Aedes aegypti was successfully infected with dengue virus or Zika virus. The proportion of virus infection by mosquitoes biting different mice (number...

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Abstract

The invention discloses application of isoretinoic acid in preparation of a product for controlling transmission of arbo-viruses and a method of the isoretinoic acid. The invention provides any one of the following applications (i)-(iii) of isoretinoic acid or pharmaceutically acceptable salts thereof: (i) an application in preparation of a product for controlling transmission of arbo-viruses; (ii) an application in preparation of a product for inhibiting the susceptibility of a transmission medium to an arthrovirus; and (iii) application in preparation of a product for reducing contact between a transmission medium and a host. It is found that by applying isoretinoic acid to the host, the efficiency of biting the host by a transmission medium can be reduced, for example, the susceptibility of the transmission medium to the arbo-viruses is inhibited, contact between the transmission medium and the host is reduced, and therefore transmission of the arbo-viruses is inhibited or blocked.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the application and method of isotretinoin in the preparation of products for controlling the spread of arboviruses. Background technique [0002] Aedes is a mosquito belonging to the Culex subfamily Aedes, commonly known as Aedes. It is distributed all over the world and is the largest genus in the mosquito family. Aedes mosquitoes can transmit many diseases and bring harm to human health. Diseases such as yellow fever and dengue fever are mainly transmitted by Aedes mosquitoes. Aedes mosquitoes are also responsible for the spread of Zika virus. Mosquitoes that have been shown to be dengue vectors include Aedes aegypti, Aedes albopictus, Aedes polynesia, etc. Among them, Aedes aegypti is a class of insects that acquire and transmit viruses by sucking blood. The arboviruses transmitted can be divided into Flaviviridae, Togaviridae and Buniaviridae. Viruses transmitted by mosquitoes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/203A61P17/02A61P31/14
CPCA61K31/203A61P17/02A61P31/14Y02A50/30
Inventor 程功朱毅斌杨学英
Owner TSINGHUA UNIV
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