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Implantable object for preventing intrauterine adhesion

A technology for intrauterine adhesions and implants, applied in the field of implantable anti-uterine adhesions, can solve ethical issues such as limited clinical application, instability, and tumor occurrence

Pending Publication Date: 2022-07-12
GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the limitations and deficiencies in the application of stem cells include: the differentiation into functional target cells (that is, directed differentiation) due to the instability of in vitro differentiation and the possibility of deregulation in the differentiation process leading to tumorigenesis, etc., tumorigenicity and possible The ethical issues involved lead to the limitation of clinical application
However, due to the huge difference in the properties of organoids and cells, there are large technical obstacles in the use of biomaterials to load organoids, and there are no related reports so far.

Method used

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  • Implantable object for preventing intrauterine adhesion
  • Implantable object for preventing intrauterine adhesion
  • Implantable object for preventing intrauterine adhesion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of endometrial organoids:

[0045] Step 1: Place the obtained mouse endometrial tissue in physiological saline containing 2% (v / v) double antibody and wash it several times with repeated shaking;

[0046] Step 2, add the washed tissue into HBSS buffer, and process it into tissue fragments of 1-2 mm3 within 15 minutes;

[0047] Step 3: Add Digestion Solution I to the tissue fragments, digest at 37°C for 1-3 h, then add HBSS buffer to terminate the digestion, and retain the precipitate after centrifugation;

[0048] Step 4: Add Digestion Solution II to the precipitate obtained in Step 3, digest at a constant temperature of 37°C for 8-12 min, add HBSS buffer to terminate the digestion, and retain the precipitate after centrifugation;

[0049]Step 5, add HBSS buffer to the pellet obtained in Step 4 to resuspend the pellet, filter with a 100 μm filter and centrifuge to obtain a pellet of endometrial epithelial cells;

[0050] Step 6, adding erythrocyte lysate t...

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Abstract

The invention relates to a cell and a biological material, in particular to an implantable material for preventing intrauterine adhesion. The invention provides an implantable material for preventing intrauterine adhesion, which comprises hydrogel and an organoid, and is an injectable implant, in the disclosure, after the implantable material which is in a liquid state at room temperature is injected into a uterine cavity, the liquid hydrogel can be shaped according to a cavity gap where the liquid hydrogel is located in a gradual solidification process at body temperature, the liquid hydrogel is shaped into a uterine cavity shape in the uterine cavity, and the liquid hydrogel is shaped into a cavity. And the contact among the wounds in the uterine cavity is completely isolated. The shaping process of the temperature-sensitive hydrogel completely meets individual requirements of different uterine cavity forms of a human body. In addition, by means of the injection type, cell exfoliation caused by the cervical canal when the uterine cavity is placed and uncertainty of the placing position are avoided.

Description

technical field [0001] The invention relates to a cell and a biological material, in particular to an implantable implant for preventing intrauterine adhesion. Background technique [0002] Intrauterine adhesions (IUA) are common factors such as miscarriage, curettage, infection, etc., which cause irreversible and deep damage to the endometrium of the basal layer of the uterus, resulting in the loss of stem cells in it, resulting in difficulty in the regeneration of the functional layer of the endometrium, and fibrosis. The scar is replaced, and the non-regenerating functional endometrium causes abnormal menstruation, and cannot provide a well-nourished, suitable environment for fertilized eggs to survive, resulting in infertility and repeated miscarriage, which is one of the important reasons for intractable infertility. At present, the traditional treatment of intrauterine adhesions includes three parts: hysteroscopic adhesion dissection (TCRA), postoperative placement of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/48A61K9/06A61P15/00
CPCA61K35/48A61K9/06A61K9/0019A61K9/0034A61P15/00
Inventor 刘丹张慧星简鹏李刚胡明月金鹤元娟娟李锋
Owner GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV