Dental pulp stem cell in-vitro culture device
A dental pulp stem cell and in vitro culture technology is applied in the field of dental pulp stem cell in vitro culturing devices, and can solve the problems of inability to realize the classification and cultivation of dental pulp stem cells, interfering with the proliferation and differentiation of dental pulp stem cells, and easily introducing external bacteria and pollutants. Achieve the effect of increasing convenience, avoiding bacteria and contaminants, and facilitating positioning
Pending Publication Date: 2022-07-12
HENAN YINFENG BIOENG CO LTD +1
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Problems solved by technology
[0004] However, the culture of dental pulp stem cells needs to be aseptic, non-toxic, rich in nutrients, suitable temperature and pH, and the required gas environment, and the above-mentioned cell culture device is cumbersome to use and easy to introduce bacteria and pollutants from the outside, which will interfere with dental pulp. The proliferation and differentiation of myeloid stem cells affect the quality of cell culture;
[0005] At the same time, it is impossible to classify and cultivate the same type of dental pulp stem cells from different sources at one time, and compare the culture of dental pulp stem cells from different sources. When the cells are inoculated, the operator does not feel good when using them, and it is not easy to sample the cells after the culture is completed. operate
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[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0036] as attached Figure 1-9 The shown device for in vitro culturing of dental pulp stem cells includes a base 1, the top of the base 1 is provided with a rotating culture mechanism, the rotating culture mechanism is provided with a crank drive assembly for driving the rotation of the rotating culture mechanism, and a rotating culture mechanism is provided with a crank drive assembly. The side is provided with a crank positioning a...
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The invention discloses a dental pulp stem cell in-vitro culture device, and particularly relates to the technical field of dental pulp stem cell culture devices.The dental pulp stem cell in-vitro culture device comprises a base, a rotary culture mechanism is arranged at the top of the base, and a crank driving assembly used for driving the rotary culture mechanism to rotate is arranged on the rotary culture mechanism; a crank positioning assembly for positioning the rotary culture mechanism is arranged on one side of the rotary culture mechanism. Through corresponding cooperative use of all the structures, the device is easy and convenient to use and operate, external bacteria and pollutants are not likely to be introduced, the cell culture quality is not likely to be affected, meanwhile, classified culture of the same category of dental pulp stem cells from different places can be achieved at a time, operation is convenient, and meanwhile the culture efficiency is improved. The subsequent single sampling operation is more convenient, and good practicability is achieved.
Description
technical field [0001] The present invention relates to the technical field of dental pulp stem cell culturing devices, and more particularly, the present invention relates to an in vitro culturing device for dental pulp stem cells. Background technique [0002] The dental pulp tissue is located in the pulp cavity inside the tooth and is the only soft tissue in the dental tissue. Through the study of human dental pulp cells, we found a kind of immunophenotype and formation that is very similar to bone marrow mesenchymal stem cells. Cells capable of mineralizing nodules are spindle-shaped in cell shape, can self-renew and differentiate in multiple directions, and have strong cloning ability. These fibroblasts isolated from dental pulp tissue are called dental pulp stem cells. [0003] Patent Application Publication No. CN 210711584 U discloses a dental pulp stem cell culture device, belonging to the technical field of cell culture, including a device main body, the bottom of ...
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IPC IPC(8): C12M3/00C12M1/10
CPCC12M23/48C12M23/52C12M37/04
Inventor 张雪梅李欢欢邱孟广王梦军程志远
Owner HENAN YINFENG BIOENG CO LTD