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Efficient preparation device and preparation method for exosome

A preparation device and exosome technology, which is applied in the field of exosome preparation, can solve the problems of long time and low exosome output, achieve mechanical and other performance enhancement, solve the effect of reducing purification effect, and reduce the consumption of manpower and material resources

Pending Publication Date: 2022-07-29
南通星中细胞工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a high-efficiency preparation device and method for exosomes to solve the problem of low output and long time in the process of exosome preparation

Method used

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  • Efficient preparation device and preparation method for exosome
  • Efficient preparation device and preparation method for exosome
  • Efficient preparation device and preparation method for exosome

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Embodiment Construction

[0069] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0070] The present invention provides such as figure 1 An efficient preparation device for exosomes, shown in , includes:

[0071] A chromatography mechanism is used to separate out the substances to be removed in the extracellular liquid through the carbon nanotube composite graphene layer to obtain semi-finished products of the outer liquid; the chromatography mechanism includes:

[0072] The first chromatography tube 11 is provide...

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Abstract

The invention discloses an efficient preparation device and a preparation method for exosomes, and belongs to the technical field of exosome preparation.The efficient preparation device comprises a chromatography mechanism for performing chromatography on substances needing to be removed in extracellular fluid through a carbon nano tube composite filter layer to obtain an external fluid semi-finished product; the purification unit is connected with the chromatography mechanism and is used for purifying the external liquid semi-finished product to obtain the exosome; a detection unit; according to the efficient preparation device and method for the exosome, flow line production is achieved, the production efficiency of the exosome is greatly improved, consumption of manpower and material resources is reduced, and the occupied area is small; the preparation time is 1 / 4 of the traditional preparation time, the efficiency is improved by 8 times, the obtaining rate is improved by two times, and 8ml of CB solution can be prepared when the exosome is collected by amniotic stem cell engineering and reaches 100ml. The chromatography effect is improved, and the influence of incomplete chromatography on the purification process is reduced; and real-time data in the chromatography process is acquired through the detection unit and is processed by the controller, so that the problem of long production period in the preparation process is solved.

Description

technical field [0001] The invention belongs to the technical field of exosome preparation, and in particular relates to an efficient preparation device and preparation method for exosomes. Background technique [0002] In 1983, exosomes were first discovered in sheep reticulocytes. Later, it was studied that a variety of cells can secrete exosomes under normal and pathological conditions, and exosomes are mainly derived from intracellular lysosomal particles. The multivesicular bodies formed by the trap are released into the extracellular matrix after the fusion of the outer membrane of the multivesicles with the cell membrane; [0003] All cultured cell types can also secrete exosomes, and exosomes are naturally present in body fluids, including blood, saliva, urine, cerebrospinal fluid, and milk. Exosomes are seen as specifically secreted membrane vesicles that participate in cells Inter-communication, the research on exosomes is increasing day by day, and the study of t...

Claims

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Application Information

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IPC IPC(8): B01D15/10B01D15/20C12M1/34C12M1/36C12M1/12C12M1/00
CPCB01D15/10B01D15/20C12M33/14C12M41/26C12M41/34C12M41/12C12M41/06C12M41/40C12M41/48C12M29/06C12M39/00
Inventor 周进王峰沈风雷袁珉芝
Owner 南通星中细胞工程有限公司
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