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Bacillus cereus S5 and application thereof

The technology of Bacillus cereus and bacterial liquid, which is applied in the field of microorganisms, can solve the problems of less research on the flavor of cigar and tobacco leaf tea, and achieve the effects of obvious reduction of irritation, obvious tea aroma, and alcohol-sucking effect.

Active Publication Date: 2022-08-09
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the use of microbial fermentation to enhance the tea flavor of cigar tobacco leaves

Method used

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  • Bacillus cereus S5 and application thereof
  • Bacillus cereus S5 and application thereof
  • Bacillus cereus S5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Bacillus cereus S5 was inoculated into the LB test tube medium, cultivated at 37 ° C overnight to obtain seed liquid, and then the seed liquid was transferred to the tea fermentation medium (Pu-erh tea leaves 20 g / L, glucose 5 g / L, sterilized at 115 ° C for 30 min. ), cultured at 37 °C for 10 h to obtain bacterial liquid.

[0044] The bacterial liquid is sprayed on the surface of the cigar tobacco leaves to be fermented, and the usage amount is 10ml / 100g of the tobacco leaves, and finally the purified water is used to make up the amount to ensure that the moisture content of the tobacco leaves is 25%. The temperature of a single fermentation cycle was raised from 25°C to 50°C and then lowered to 25°C, and the humidity was increased from 70% relative humidity to 85% and finally decreased to 70%. Each fermentation cycle is 6d, and a total of 5 fermentation cycles are required.

[0045] The tea medium without Bacillus cereus S5 (20g / L of Pu'er tea, 5 g / L of glucose, steri...

Embodiment 2

[0051] Bacillus cereus S5 was inoculated into the LB test tube medium, cultivated at 37 ° C overnight to obtain seed liquid, and then the seed liquid was transferred to the tea fermentation medium (Pu-erh tea leaves 20 g / L, glucose 5 g / L, sterilized at 115 ° C for 30 min. ), cultured at 37 °C for 10 h to obtain bacterial liquid.

[0052] The bacterial liquid is sprayed on the surface of the cigar tobacco leaves to be fermented, and the usage amount is 15ml / 100g of the tobacco leaves, and finally, it is filled with purified water to ensure that the moisture content of the tobacco leaves is 27%. The temperature of a single fermentation cycle was raised from 25°C to 50°C and then lowered to 25°C, and the humidity was increased from 70% relative humidity to 85% and finally lowered to 70%. Each fermentation cycle is 4 days, and a total of 7 fermentation cycles are required.

[0053] The tea culture medium without Bacillus cereus S5 (20g / L of Pu'er tea, 5 g / L of glucose, sterilized...

Embodiment 3

[0059] Bacillus cereus S5 was inoculated into the LB test tube medium, cultivated at 37 ° C overnight to obtain seed liquid, and then the seed liquid was transferred to the tea fermentation medium (Pu-erh tea leaves 20 g / L, glucose 5 g / L, sterilized at 115 ° C for 30 min. ), cultured at 37 °C for 10 h to obtain bacterial liquid.

[0060] The bacterial liquid was sprayed on the surface of the cigar tobacco leaves to be fermented, and the usage amount was 15ml / 100g of the tobacco leaves, and finally, purified water was used to make up the amount of the tobacco leaves to ensure that the moisture content of the tobacco leaves was 30%. The temperature of a single fermentation cycle was raised from 25°C to 50°C and then lowered to 25°C, and the humidity was increased from 70% relative humidity to 85% and finally decreased to 70%. Each fermentation cycle is 3d, and a total of 8 fermentation cycles are required.

[0061] The tea culture medium without Bacillus cereus S5 (20g / L of Pu'...

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PUM

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Abstract

The invention discloses a bacillus cereus S5 and an application thereof. The preservation number of the bacillus cereus S5 is CGMCC (China General Microbiological Culture Collection Center) NO.24815. The bacillus cereus S5 has the advantages that the bacillus cereus S5 According to the method, the cigar tobacco leaves are fermented by using a tea aroma microorganism-bacillus cereus S5, so that the cigar tobacco leaves with obvious tea aroma can be obtained. The cigar tobacco leaves fermented by the bacillus cereus S5 are obvious in tea fragrance, the cigar is smoked more mellowly, and the irritation is obviously reduced.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, and more particularly, to a Bacillus cereus S5 and its application. Background technique [0002] Cigars are an ancient tobacco product with a rich and varied aroma that is loved by some people. In recent years, the sales and sales of domestic cigars have increased significantly, and the market space is huge. The development of cigars with various styles has a more stimulating effect on the cigar market. Fermentation is a key step in the formation of the style of cigar tobacco leaves, and the type of microorganisms plays a decisive role in the fermentation process of cigar tobacco leaves. [0003] Pu-erh tea is a famous fermented tea in Yunnan, which is loved by the people for its mellow taste, amber color and special aroma. Combined with the style of Pu'er tea, the use of microbial fermentation technology to obtain tea-flavored cigars is of great value to enrich the cigar catego...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/085
CPCC12N1/205C12N1/20A24B15/20C12R2001/085Y02W30/40
Inventor 段如敏吴丽君刘晶王凯阴耕云王磊尹志豇田然刘馨芮彤白晓莉卢伟
Owner CHINA TOBACCO YUNNAN IND
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