Micro-array chip

Abstract

A micro array chip is formed by high-speed injection molding. A chip body has a thickness of 1 mm and each well has a capacity of 1.2 muL with the bottom having a wall thickness of 250 mum. An opening of each well is surrounded by an annular protrusion part, which is raised from a surface of the chip body by a height of 200 mum. The openings of wells may be closed with a sheet of sealant made of aluminum or a resin. An overall shape of the micro array chip is rectangular and is a flat plate with a level bottom surface. Therefore, the micro array chip can be used with a heat block in flat plate form, which is independent of chip specifications such as a number of wells and their shape.

Description

technical field [0001] The present invention relates to a microarray chip having wells for a reaction container in which a living sample is subjected to enzyme treatment, derivative formation, PCR (polymerase chain reaction), Sanger's reaction, etc., More specifically, it relates to microarray chips for small-capacity reaction vessels. Background technique [0002] When polymerase chain reaction (hereinafter referred to as PCR) and other reactions are performed on a small amount of samples, a microtiter plate having 96 or 384 wells is conventionally used as a reaction vessel. [0003] For reactions in microtiter plates, the sample volume required is 20 to 40 microliters for a 96-well plate and 5 to 10 microliters for a 384-well plate. [0004] With recent improvements in the performance of DNA sequencers and other equipment, small amounts of samples can be analyzed. As a result, pretreatments such as PCR and Sanger reactions can now be performed on small sample quantities....

AI Technical Summary

Problems solved by technology

Therefore, when using microtiter plates with different numbers of wells, it is inconvenient to change the heating block according to the microtiter plate

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