Multiple PCR quickly investigating method for avian influenza virus

A technology of avian influenza virus and detection method, which is applied in the detection field of identifying avian influenza H5 and H9 subtype viruses, can solve the problems of long time for reporting results and heavy workload, and achieve the effect of rapid identification and serological typing test methods.

Inactive Publication Date: 2006-02-22
江苏瑞邦生物科技有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, in the work of laboratory diagnosis, the classic method stipulated by the World Organization for Animal Health (OIE) is virus isolation, identification and serological typing test. This method takes a long time to report the results and has a large workload.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0008] 1. Primer design:

[0009] Three pairs of specific primers (NP-Forward, NP-Reverse; H 5 A-Forward, H 5 A-Reverse; H 9 A-Forward, H 9 A-Reverse), the above three pairs of specific primers were synthesized by Shanghai Boya Biological Co., Ltd. The size of the expected amplified target fragment is divided into NP: 330bp; H 5 A: 545bp; H 9 A: 488bp.

[0010] NP-Forward: 5’-[CAG ATA TTG GGC TAT AAG GAC]-3’

[0011] NP-Reverse: 5’-[GCA TTG TCT CCG AAG AAA TAA G]-3’

[0012] h 5 A-Forward: 5’-[ACA CAT GCT CAG GAC ATA CT]-3’

[0013] h 5 A-Reverse: 5’-[CTC TGA TTC AGT GTT GAT GT]-3’

[0014] h 9 A-Forward: 5’-[CTC CAC ACA GAG CAC AAT GG]-3’

[0015] h 9 A-Reverse: 5’-[GTT GTC ACA CTT GTT GTT GT]-3’

[0016] 2. Multiplex RT-PCR:

[0017] 1) Amplification of the virus: Inoculate the avian influenza virus in the allantoic cavity of the 10-day-old non-immune chicken embryo, collect the allantoic fluid of the dead chicken embryo after 36-72 hours, and detect its hema...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a verification virus, especially relates to a detecting method for fowl grippe H5, H9 subtype virus. It uses Trizol kit to extract the sample gene RNA, and then carries on reverse rerecording and synthesizes into cDNA, then disperses several genes special expanded in one-time PCR, finally carries on electrophoresis verification. Because the special gene quantity is small in single suspected case sample, the invention forms large amount of gene replicated segments through rerecording the RNA in sample into cDNA, then expanding the NP, H5A, H9A genes in one time, thus the method is convenient, it can judge if it is fowl grippe or not, and judges the which subtype does it belong to in H5 or H9. The invention is quick and accurate.

Description

technical field [0001] The invention discloses a detection method for identifying viruses, especially avian influenza H5 and H9 subtype viruses. Background technique [0002] Highly pathogenic avian influenza (High1y Pathogenic Avian Influenza, referred to as HPAI), once known as fowl plague, is an acute and severe infectious disease caused by a specific subtype of influenza A virus in the family Orthomyxoviridae, which seriously endangers the poultry industry and human beings. healthy. The World Organization for Animal Health (OIE) lists it as a class A infectious disease, and my country defines it as a class I animal infectious disease. [0003] Since the end of 2003, H5N1 and other subtypes of avian influenza have successively occurred in South Korea, Vietnam, Thailand, Japan, Indonesia, Pakistan, Laos, Cambodia, and Taiwan. The rapid spread of the epidemic and the serious loss are really rare. And our country has not been spared, and epidemics have occurred in more tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/17
Inventor 焦新安耿士忠潘志明黄金林孙林殷月兰张苏华孙泉云
Owner 江苏瑞邦生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap