Efficient hybrid rice false removing method
A hybrid rice and seed technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problem of not solving the problem of yield loss, and not having the ability to eliminate false hybrids, and achieve the effect of improving purity and ensuring purity.
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Embodiment 1
[0012] High-Efficiency Elimination of Fakes in the Propagation of Hybrid Rice CMS Lines
[0013] Take the two-line hybrid rice CMS line Yutu S with albino-to-green leaf color markers as an example. The breeding technique of Yutu S with albino mark is the same as that of ordinary green two-line hybrid rice male sterile line. The CMS with albino mark can be used to increase the purity of the CMS line: in the breeding field, the seedlings with albino mark are used as pure seedlings of Yutu S, and the seedlings of other colors are mixed seedlings, all of which are removed, so as to breed high-purity The Yutu S seeds.
Embodiment 2
[0015] High-efficiency false removal in the process of hybrid rice seed production
[0016] Taking the female parent of the two-line hybrid rice male sterile line Yutu S with albino leaf color markers and the hybrid rice restorer line 9311 as the male parent, the production of hybrid seeds was prepared as an example. Seed production techniques for Yutu S with albino markers are the same as those for common green two-line hybrid rice male sterile lines. During the seed production of Jade Rabbit S and 9311, among the seedlings of the male sterile line Jade Rabbit S, the seedlings with albino marks were used as pure Jade Rabbit S seedlings, and the seedlings of other colors were hybrid plants, which were all removed, so as to ensure the purity of the hybrid female parent. Prepare high-purity hybrid rice seeds.
Embodiment 3
[0018] Efficient false removal during hybrid rice production:
[0019] For the hybrid rice seeds used in the field, if the seedlings with albino marks are manually removed at the seedling stage and the normal green seedlings are retained, high-purity hybrid rice can be planted and rice yield can be increased. In 2002, the hybrid combination "Yueyou 1" F with Yutu S and NHR1 containing 9.7% 1 For the seedlings, the measures of manually removing the albino seedlings in the seedling fields were adopted, and no Jade rabbit S plants were found in the mature stage.
[0020] When hybrids prepared with albino marked CMS lines are used in the field, the growth competition of albino seedlings is significantly weaker than that of hybrids, and false hybrids (especially albino self-bred seedlings) are automatically eliminated by virtue of growth competition before transplanting or automatically eliminated during seedling pulling. , to provide high-purity "safe" seeds for hybrid rice produ...
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