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Method or improving alkaline pectase enzyme activity in preparing alkaline pectase process by fermenting method

A technology of pectinase enzymatic activity and fermentation process, which is applied in the field of biological enzyme preparation, can solve the problems of cost, has not been widely used, etc., and can shorten the fermentation period, improve the environment and the quality of cotton textile products, and the operation method is simple and convenient. Effect

Inactive Publication Date: 2006-08-30
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to cost issues, it has not been widely used so far.

Method used

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  • Method or improving alkaline pectase enzyme activity in preparing alkaline pectase process by fermenting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Inoculate 1ml of the bacterial suspension preserved at -70°C into the seed culture medium, fill 50ml of the seed culture medium in a 500ml triangular flask, cultivate it on a rotary shaker at 37°C and 200rpm for 14 hours, and then follow the steps of 8 The inoculum size of % is inoculated in the 7L fermenter, and the fermenter with the liquid loading capacity of the fermentation medium is 4L of the fermentation medium in the fermenter of 7L. The fermentation conditions are: fermentation temperature 37°C, aeration rate 4L / min, and stirring speed 600rpm to control K L a is 350h -1 , The fermentation device adopts an automatic defoaming system. The whole process adopts the method of regular sampling to analyze various parameters of the fermentation broth offline, including parameters such as enzyme activity, dry weight of bacteria and amount of reducing sugar. Under this condition, the fermentation period is 8 hours, and the final enzyme activity of the fermentation reac...

Embodiment 2

[0029] Change the stirring speed to 500rpm to control K L a is 200h -1 , all the other conditions are the same as in Example 1. Under this operating condition, the fermentation period is 12 hours, and the final enzyme activity of fermentation is 39U / ml. See the fermentation process figure 1 .

Embodiment 3

[0031] Change the stirring speed to 700rpm to control K L a is 500h -1 , all the other conditions are the same as in Example 1. Under this operating condition, the fermentation period is 12 hours, and the final enzyme activity of fermentation is 34U / ml. See the fermentation process figure 1 .

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Abstract

The method of raising the alkaline pectase enzyme activity during alkaline pectase preparing fermentation process belongs to the field of enzyme preparing technology. By means of controlling the stirring speed and thus the oxygen transferring speed in fermenting liquid to obtain proper volume oxygen dissolving coefficient (KLa) and deep liquid fermentation, alkaline pectase with enzyme activity as high as 40 U / ml is prepared. The said process has high enzyme activity of alkaline pectase enzyme, low cost, short fermentation period, simple operation and easy industrial application. The prepared alkaline pectase has polygalacturonase as main component and may be used in the refining treatment of cotton fabric in improved environment and raised product quality.

Description

technical field [0001] The invention discloses a method for increasing the enzyme activity of alkaline pectinase in the process of preparing alkaline pectinase by fermentation, belonging to the technical field of biological enzyme preparation. The invention relates to a method of controlling the transfer rate of oxygen in the fermentation liquid during the fermentation process-volume dissolved oxygen coefficient K L The a value, the method for improving the activity of alkaline pectinase, obtains the alkaline pectinase with high enzyme activity. Background technique [0002] Pectinase (pectinases) is a kind of enzyme system that can decompose pectin (a straight-chain polymer formed by D-galacturonic acid linked by α-1,4 glycosidic bonds) in plant tissues, alkaline Pectinase refers to pectinase with relatively high activity in the alkaline range, and generally refers to polygalacturonate lyase (PGL). [0003] At present, acid pectinase has been widely used in the extraction...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/24C12R1/07D06M16/00D06M101/04
Inventor 陈坚张健红堵国成
Owner JIANGNAN UNIV