High yield fungus for alkali pectinase and screen method as well as ferentation method for producing alkali pectinase by using the strain
A pectinase and alkaline technology, which is applied in the direction of bacteria, hydrolytic enzymes, textiles and papermaking, etc., can solve the problems of unsatisfactory results, no alkaline pectinase-producing bacteria screening, etc., to improve the environment and product quality, The effect of changing the scouring process
Inactive Publication Date: 2004-03-10
JIANGNAN UNIV
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Problems solved by technology
Although a series of enzymes developed by foreign companies have been reported to have a good application in foreign countries, due to the large differences in the quality of cotton in various production areas, the effect in our domestic cotton textile processing and use is not satisfactory, and it has not been very successful. good promotion
At the same time, there are no domestic research reports on the application of self-developed alkaline pectinase and the screening of its producing bacteria.
Method used
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Embodiment Construction
[0017] Scrape the two-ring strains from the inclined surface, place them in a 250ml Erlenmeyer flask with 25ml seed medium for seed culture. The conditions are that the incubation time is 12 hours, the temperature is 37° C., and the rotation speed of the shaker is 200 rpm. Then, the 10% inoculum was inserted into a 250ml Erlenmeyer flask equipped with 25ml fermentation medium for fermentation. The conditions are as follows: the incubation time is 32 hours, the temperature is 37° C., and the rotation speed of the shaker is 200 rpm. A fermentation broth with an enzyme activity of 20u / ml can be obtained.
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A Bacillus pumilus (CCTCC No.M203042) for preparing the alkaline pectinase wigh high ouput is disclosed. It is obtained by the plate transparent ring screening of putrescent tangerine peel. Said alkaline pectinase used for refining cotton fabrics is prepared through deep fertilization with said Bacillus pumilus.
Description
technical field [0001] The invention relates to a high-yielding alkaline pectinase bacterium, a screening method thereof, and a fermentation method for producing alkaline pectinase by using the strain, belonging to the technical field of bioengineering, in particular to a bacillus strain with high production of alkaline pectinase. Background technique [0002] Due to the natural impurities in cotton fiber, the traditional cotton textile dyeing and finishing process needs to consume a lot of water and chemical substances, which not only consumes resources, but also seriously pollutes the environment, which is no longer suitable for the development of the whole society. The way to solve this problem is to develop an environment-friendly dyeing and finishing process, that is, a green dyeing and finishing process, and the application of biotechnology is one of the important ways. [0003] In the process of cotton textile processing, the application of biotechnology is becoming m...
Claims
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Login to View More IPC IPC(8): C12N1/20C12N9/24C12S11/00D06M16/00
Inventor 陈坚堵国成董云舟伦世仪
Owner JIANGNAN UNIV