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DREB transcription factor and its coding gene and use

A technology of transcription factors and response elements, which can be used in applications, genetic engineering, plant genetic improvement, etc., can solve the problems of cold resistance and low salt and alkali tolerance, and achieve great economic value, wide application prospects, and plant resistance. The effect of inverse characteristic improvement

Inactive Publication Date: 2006-12-20
TSINGHUA UNIV
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Problems solved by technology

Tall fescue (Festuca arundinacea Schreb.) has excellent heat resistance, drought resistance, and disease resistance, and is an excellent turfgrass widely planted in recent years, but its low cold resistance and salt tolerance limit its application in the harsh western environment. to promote

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  • DREB transcription factor and its coding gene and use
  • DREB transcription factor and its coding gene and use
  • DREB transcription factor and its coding gene and use

Examples

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Embodiment Construction

[0029] A kind of tall fescue DREB class transcription factor proposed by the present invention and its coding gene combination embodiment and accompanying drawing are described in detail as follows (methods used in the following examples all adopt the third edition of "Molecular Cloning Experiment Guide" if no special instructions The general method exposed in ):

[0030] The embodiment of the preparation method of the FaDREB1-encoding gene of the present invention includes two parts: the separation of the FaDREB1 gene and the expression pattern of the FaDREB1 gene under cold, salt and drought-induced treatment; wherein, the separation of the FaDREB1 gene specifically includes the following steps:

[0031] 1. Construction of tall fescue cold-induced cDNA library

[0032] 1) Soak the seeds of Gramineae Tall Fescue (Festuca arundinacea Schreb.) in 1% sodium hypochlorite solution for disinfection for 20 minutes, wash with sterile water three times, and sow in 1 / 2MS solid medium (...

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Abstract

Gaoyangmao drought responder element combined protein transcription factor, its coding gene and use are disclosed. The transcription factor is protein with amino acid residue sequence in sequential table SEQ ID No: 1, it has regulating protein with transcription and activation functions. The coding gene is nucleotide sequence in sequential table SEQ ID No: 2; or nucleotide sequence of protein sequence in sequential table SEQ ID No: 2; or it has homology above 95% with nucleotide sequence defined by SEQ ID No: 2 in sequential table, and protein nucleotide sequence for regulating anti-reverse performance with coding transcription and activation functions; or nucleotide sequence to hybridize with DNA sequence defined by SEQ ID No: 2 in sequential table under strict condition. It can be used to improve anti-reverse performance of plant.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, in particular to a tall fescue drought response element binding protein (DREB) type transcription factor and its coding gene and its application in cultivating plants with improved stress resistance. technical background [0002] Transcription factors are proteins that bind to the cis-acting elements in the gene promoter region in organisms to regulate the expression of the gene. They play a very important role in the life activities of organisms. They not only participate in the growth and development of organisms, but also regulate the response of organisms to external adversities such as drought, high salinity, low temperature, and pathogen infection. Yamaguchi-Shinozaki and Shinozaki found a stress-responsive cis-acting element, the drought-responsive element (DRE, drought-responsive element), in the study of the promoter region of the stress-responsive gene rd29A. Liu Qiang e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29A01H1/00
Inventor 阳文龙刘强公衍道赵南明
Owner TSINGHUA UNIV
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