Supercharge Your Innovation With Domain-Expert AI Agents!

DREB transcription factor and its coding gene and use

A technology of transcription factors and response elements, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of cold resistance and salt-alkali tolerance, low limit promotion, etc., achieve huge economic value, wide application prospects, plant resistance The effect of inverse characteristic improvement

Inactive Publication Date: 2005-10-12
TSINGHUA UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tall fescue (Festuca arundinacea Schreb.) has excellent heat resistance, drought resistance, and disease resistance, and is an excellent turfgrass widely planted in recent years, but its low cold resistance and salt tolerance limit its application in the harsh western environment. to promote

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DREB transcription factor and its coding gene and use
  • DREB transcription factor and its coding gene and use
  • DREB transcription factor and its coding gene and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] A kind of tall fescue DREB class transcription factor proposed by the present invention and its coding gene combination embodiment and accompanying drawing are described in detail as follows (methods used in the following examples all adopt the third edition of "Molecular Cloning Experiment Guide" if no special instructions The general method exposed in ):

[0030] The embodiment of the preparation method of the FaDREB1-encoding gene of the present invention includes two parts: the separation of the FaDREB1 gene and the expression pattern of the FaDREB1 gene under cold, salt and drought-induced treatment; wherein, the separation of the FaDREB1 gene specifically includes the following steps:

[0031] 1. Construction of tall fescue cold-induced cDNA library

[0032] 1) Soak the seeds of Gramineae Tall Fescue (Festuca arundinacea Schreb.) in 1% sodium hypochlorite solution for 20 minutes to disinfect, wash with sterile water three times, and sow on 1 / 2 MS solid medium (pH5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Gaoyangmao drought responder element combined protein transcription factor, its coding gene and use are disclosed. The transcription factor is protein with amino acid residue sequence in sequential table SEQ ID No: 1, it has regulating protein with transcription and activation functions. The coding gene is nucleotide sequence in sequential table SEQ ID No: 2; or nucleotide sequence of protein sequence in sequential table SEQ ID No: 2; or it has homology above 95% with nucleotide sequence defined by SEQ ID No: 2 in sequential table, and protein nucleotide sequence for regulating anti-reverse performance with coding transcription and activation functions; or nucleotide sequence to hybridize with DNA sequence defined by SEQ ID No: 2 in sequential table under strict condition. It can be used to improve anti-reverse performance of plant.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, in particular to a tall fescue drought response element binding protein (DREB) type transcription factor and its coding gene and its application in cultivating plants with improved stress resistance. technical background [0002] Transcription factors are proteins that bind to the cis-acting elements in the gene promoter region in organisms to regulate the expression of the gene. They play a very important role in the life activities of organisms. They not only participate in the growth and development of organisms, but also regulate the response of organisms to external adversities such as drought, high salinity, low temperature, and pathogen infection. Yamaguchi-Shinozaki and Shinozaki found a stress-responsive cis-acting element, the drought-responsive element (DRE, drought-responsive element), in the study of the promoter region of the stress-responsive gene rd29A. Liu Qiang e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H1/00C07K14/415C12N15/29
Inventor 阳文龙刘强公衍道赵南明
Owner TSINGHUA UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com