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Fabrication method and application for patulin immune chromatography detection test paper

A technology for immunochromatographic detection and patulin, which is applied to measuring devices, analytical materials, instruments, etc., to achieve the effects of high sensitivity, easy operation, and low cost

Inactive Publication Date: 2007-02-14
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of immunochromatography for the detection of patulin has not been reported yet

Method used

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  • Fabrication method and application for patulin immune chromatography detection test paper
  • Fabrication method and application for patulin immune chromatography detection test paper
  • Fabrication method and application for patulin immune chromatography detection test paper

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0034] A patulin immunochromatographic detection test paper, comprising a liner 1, a sample pad 2, a tracer particle conjugate pad 3, a nitrocellulose membrane 4, an absorbent pad 5, a detection line 6, a quality control line 7, and the liner 1 There are sample pad 2, tracer particle conjugate pad 3, nitrocellulose membrane 4, and water-absorbing pad 5 in sequence; tracer particle conjugate pad 3 has anti-patulin antibody 10 labeled with tracer particle 9, and nitrocellulose The prime film 4 is also provided with a detection line 6 and a quality control line 7, the detection line 6 has a patulin detection antigen 8, and the quality control line 7 has an anti-antibody 11, wherein the anti-antibody 11 is an antibody against the patulin antibody 10 .

[0035] The detection principle is as follows: sample 12 is added dropwise on the sample pad 2, if there is patulin in the sample 12, the patulin will move to the tracer particle conjugate pad 3 through chromatography, and the limit...

Embodiment 2

[0038] The tracer particles 9 are colloidal gold particles. All the other are with embodiment 1.

Embodiment 3

[0040] The tracer particles 9 are latex particles. All the other are with embodiment 1.

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Abstract

This invention discloses a penicillium patulum immune chromatography test paper and its process method and utility. The test paper comprises pad, sample pad, tracing particle compound pad, pyroxylin film, adsorption pad, test thread, and control thread. The method is the following: to orderly put sample pad, tracing particle compound pad, pyroxylin film and adsorption pad on the pad; the tracing particle compound pad has penicillium antibody with tracing particle mark; the pyroxylin film has test thread and control thread; the test thread has penicillium patulum antigen.

Description

technical field [0001] The invention belongs to the field of biotechnology, and discloses a preparation method and application of a patulin immunochromatographic detection test paper. Background technique [0002] Patulin, PAT is a toxic metabolite produced by fungi, first discovered and purified by Glister in 1941. Experiments found that patulin is a broad-spectrum antibiotic, but subsequent studies confirmed that it has strong toxicity to experimental animals. Since then, the research on patulin has shifted to its toxicity and pollution to food and feed. [0003] There are 16 species of fungi belonging to 3 genera, such as Penicillium diexpansus, Penicillium patulin, Penicillium clavum, Penicillium soil, etc., which can produce patulin. The survey found that some patulin-producing bacteria are widely distributed in nature, often causing apples, hawthorns, etc. Such as fruit mildew, rice and feed mildew. In 1995, Song Jiayu et al. tested apples, hawthorns and their produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/531
Inventor 许杨邓舜洲赖卫华陈高明熊勇华
Owner WUXI ZODOLABS BIOTECH