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Manipulation of tocopherol levels in transgenic plants

A technology for transgenic plants and tocopherols, applied in the field of changing the composition of plant tocopherols, heterologous gene constructs, and transgenic plants, can solve the problems of expensive supplements, unwillingness to take vitamin supplements regularly, etc.

Inactive Publication Date: 2001-11-28
内华达州立大学
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Additionally, supplements are quite expensive and the average person is unwilling to take vitamin supplements on a regular basis

Method used

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  • Manipulation of tocopherol levels in transgenic plants
  • Manipulation of tocopherol levels in transgenic plants
  • Manipulation of tocopherol levels in transgenic plants

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Embodiment

[0049] Arabidopsis and Synechocystis as complementary model systems for studying and manipulating tocopherol biosynthesis in plants

[0050] Complementary molecular genetic approaches were implemented in parallel in Arabidopsis thaliana and Synechocystis strain PCC6803 to isolate and characterize the Synechocystis 2-methyl-6-chloroplastoquinone / 2-methyl- 6-solanoid plastomaquinone-9 methyltransferase. These two model organisms were chosen because both synthesize tocopherol through the same pathway, and both are easily controlled genes, molecules, and bioactive systems. Many reactions and pathways are similar in the two organisms, most relevant to this patent is the similarity in the synthesis of tocopherol. The ease of gene function in Synechocystis to be tested by genetic disruption, combined with the recently reported complete Synechocystis genome sequence (27), makes Synechocystis an excellent candidate for isolating tocopherol (synthetic) pathway enzymes. model system an...

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Abstract

Disclosed is a gene sequence which as been identified as encoding 2-methyl-6-phytylplastoquinol / 2-methyl-6-solanylplastoquinol-9 methyltransferase from photosynthetic organisms. The enzyme 2-methyl-6-phytylplastoquinol / 2-methyl-6-solanylplastoquinol-9 methyltransferase is a fundamental enzyme in the production of tocopherols and plastoquinols in higher plants. 2-Methyl-6-phytylplastoquinol / 2-methyl-6-solanylplastoquinol-9 methyltransferase is believed to be involved in regulating the relative amounts of the various tocopherols present in photosynthetic organisms. By introducing a genetic construct having a 2-methyl-6-phytylplastoquinol / 2-methyl-6-solanylplastoquinol-9 methyltransferase coding sequence placed under the control of a plant promoter in a plant, transgenic plants can be made having altered 2-methyl-6-phytylplastoquinol / 2-methyl-6-solanylplastoquinol-9 methyltransferase expression, to effect changes in the tocopherol profile of the plant. Transgenic plants can be made that have altered tocopherol levels in their tissues.

Description

[0001] Related Application Cross Documentation [0002] This application claims priority to US Provisional Application No. 60 / 097,863, filed August 25,1998. [0003] Statement Regarding Federally Sponsored Research or Development [0004] Not applicable. Background of the invention [0005] Vitamin E is an essential component of the diet of mammals. Epidemiological evidence suggests that vitamin E supplementation reduces the risk of cardiovascular disease and cancer, aids immune function, and generally prevents or slows the progression of many degenerative diseases in humans (Traber and Sies, Annu.Rev.Nutr.16 :321-347, 1996). Vitamin E acts to stabilize the phospholipid bilayers of biological membranes (Skrypin and Kagan, Biochim. Biophys. Acta 815:2091995; Kagan, N.Y. Acad. Sci. 121, 1989; Gomez Femandez et al., Ann. N, Y, Acad. Sci.109 page, 1989), reduce the polyunsaturated fatty acid (PUFA) free radical (the people such as Fukuz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N5/10C12N9/10C12N15/09C12N15/82C12P17/06C12R1/91
CPCC12N9/1007C12N15/8243
Inventor D·德拉彭娜
Owner 内华达州立大学
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