Healthy product raw liquor of armilariella tabescens for strengthening the body and protecting the liver and method for improving the formulation of armilariella tabescens
A technology for health care products and brightening bacteria, which is applied in the fields of bioengineering technology and pharmaceutical technology, can solve the problems of inability to achieve industrialized large-scale production, difficult to industrialized-scale production, large dosage of oral liquid, etc., so as to improve the prevention and treatment of chemical liver damage. , The effect of preparing many dosage forms and long shelf life
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example 1
[0041] Preparation of Leucobacteria stock solution
[0042] 1. The strains used are the strains [Armillariella tabescens (Scop.ex Fr.) Sing] from Beijing Microorganism Culture Collection Center
[0043] 2. Bacteria slant culture: Aseptically inoculate the purchased strains on a PDA slant, and put them into a 28°C incubator for 10-15 days.
[0044] 3. Preparation of shake flask liquid seeds: cut the cultured slant strains and inoculate them into 250ml or 500ml Erlenmeyer flasks filled with liquid medium, place them in a shaker at 28°C and 140rpm and culture them in the dark for 7 days. The above-mentioned liquid seed medium is: 20% of potato juice, 2% of glucose, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate, V b1 A small amount, pH6.0.
[0045] 4. Bright bacteria fermentation: inoculate the liquid seeds with 10% of the inoculum amount of the fermentation medium, and place them on a shaker at 24° C. and 140 rpm for 6 days. The fermentation medium is: glu...
example 2
[0049] Preparation of Liangjun Granules
[0050] 20% starch, 20% dextrin, 10% sucrose powder and other auxiliary materials are added to the stock solution, and after mixing, the above slurry is spray-dried. The inlet temperature of spray drying is 110-150°C, and the outlet temperature is 50-60°C. Sieve through 40-60 mesh, pack separately. The main technical indicators are as follows:
[0051] Effective polysaccharide 22.1%, protein 15.6%, polypeptide 11.3%, moisture 4.3%.
example 3
[0053] Preparation of Leucobacteria Tablets
[0054] Concentrate the bright bacteria stock solution to 1 / 10, add 75% three-fold ethanol to precipitate overnight, collect the precipitate, and wash it successively with acetone and ether to obtain a dry powder. According to the weight ratio, 40% of Leucobacteria powder, 30% of starch, 20% of dextrin, and 10% of sucrose are mixed evenly, and 95% ethanol is used as a wetting agent, passed through a 20-mesh sieve to make wet granules, and dried in an oven at 60°C until the moisture content is 5%. , Whole grain 20 mesh, add 3% talcum powder and compress into tablets. Each piece weighs 0.4g. The main technical indicators of the product are as follows:
[0055] Effective polysaccharide 26.5%, protein 17.8%, polypeptide 12.1%, moisture 4.1%.
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