Additive for photosyntheric bacterial culture medium

A technology of photosynthetic bacteria and culture medium, which is applied in the direction of bacteria, adding compounds to stimulate growth, etc., which can solve the problems of low bacterial content, unsatisfactory production and application, slow growth and reproduction of bacterial strains, etc., and achieve good adaptability and ecological Environmental friendliness and high bacterial concentration in the product

Inactive Publication Date: 2003-04-30
WEIHAI GOLD FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of the conventional culture method are that the growth and reproduction of the strain are slow and the bacteria content is small, which is far from meeting the requirements of production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Follow these steps:

[0025] (1) Seed mother activation: the seed mother of photosynthetic bacteria is activated (transplanted to a new slope, or transferred to a liquid culture medium for 3 to 5 days); wherein the culture medium that adds the additive of the present invention needs 3 days to reach Use requirements, adopting the culture medium that does not add additive of the present invention then needs 5 days;

[0026] (2) Seed fermentation and cultivation: insert the good seed mother of photosynthetic bacteria activation into the seed fermentation medium, dress in a 2000 ml transparent glass bottle (the amount of medium is 70%, and the balance is the inoculation amount), and place Cultivate under natural sunlight (temperature at 20-30°C), the seed medium matures in 7 days, the bacteria content reaches 200 million / ml, the culture medium is bright red or deep red, and the photosynthetic bacteria are lively and neat when checked under a microscope. It can be used for ...

Embodiment 2

[0033] The present invention can be operated according to the following steps:

[0034] (1) seed mother activation: with embodiment 1 (1);

[0035] (2) seed fermentation culture: with embodiment 1 (2);

[0036] (3) Enlarged fermentation culture:

[0037] Photosynthetic bacteria seed fermentation culture liquid (the number of active photosynthetic bacteria reaches 200,000,000 per ml) is transferred to the expanded fermentation medium by 30% and multiplied. This expanded fermentation medium is selected from soybean waste water, at 10 kg 7 kg of soybean product waste water in a white plastic bucket, (wherein the composition of additives is: 1.0 g / liter of sodium propionate, 0.001 g / liter of ferrous sulfate, and 0.5 g / liter of yeast extract.) The white Plastic barrels can be cultivated under natural sunlight and mature in 10 days, and its technical indicators can reach the following table 1.

[0038] pH

Embodiment 3

[0040] The present invention can be operated according to the following steps:

[0041] (1) seed mother activation: with embodiment 1 (1);

[0042] (2) seed fermentation culture: with embodiment 1 (2);

[0043] (3) Enlarged fermentation culture (under laboratory conditions): inoculation and cultivation method are the same as embodiment 1 (3);

[0044] Under laboratory conditions, the fermentation medium that adds additives is optimized, and 350 milliliters of additive-free culture mediums are filled in 500 milliliters of bottles, and additives of different combinations are added (see uniform design scheme table 2 for its addition), and 9 are established in total. Process, numbered as 1, 2, 3, 4, 5, 6, 7, 8, 9 and then inoculate 30% of photosynthetic bacteria seed solution in every bottle, put it under natural sunlight and cultivate it (temperature 25~30 ℃) for 10 days, measure The number of photosynthetic bacteria.

[0045] Numbering

1

2

3

4

...

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PUM

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Abstract

An additive for the culture medium of photosynthesizing bacteria contains sodium propionate and iron ion salt (10-100 PPM). Its advantages are high reproduction speed, low cost, and friendly to ecological environment.

Description

technical field [0001] The invention relates to a method for cultivating photosynthetic bacteria, in particular to an additive for photosynthetic bacteria medium. technical background [0002] Photosynthetic bacteria (Photosynthetic Bacteria referred to as PSB) ecological distribution is very wide, mainly distributed in oceans, rivers, lakes, activated sludge and soil, is a kind of aquatic and hydrosphere microorganisms. Photosynthetic bacteria use sunlight as energy source, sodium acetate, sodium lactate, mannitol, malic acid, citric acid, glucose, glycerin and other organic substances as carbon and nitrogen sources, and the bacteria have fast growth and reproduction speed, wide and easy source of medium Its products are widely used in feed industry, agricultural production, environmental protection and many other aspects; therefore, it has been paid more and more attention by scientific and technological workers and business people; in previous studies, people often Focus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38
Inventor 薛德林胡江春马成新王伟刘军王书锦
Owner WEIHAI GOLD FEED
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