Tumour cell drug sensitive testing method and its testing plate

A tumor cell and detection method technology, applied in the field of tumor cell drug susceptibility detection and its detection board, can solve the problems of serious patient body, affect colorimetric results, aggravate toxic effects, etc., and achieve simplified operation procedures, simple operation, and extended storage. period effect

Inactive Publication Date: 2003-07-16
崔克勤
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Problems solved by technology

[0005] 3. Selection of anticancer drugs and avoidance of toxicity. Anticancer drugs kill normal tissue cells as well as tumor cells. Due to the blindness and drug resistance of chemotherapy, when doctors try to use large doses, multiple drugs, and shorten the course of treatment, etc. When the method improves the curative effect, the toxic effect will be further aggravated, causing serious or even fatal damage to the patient's body.
Moreover, due to many steps and a long time, the operation will inevitably be disturbed by human factors. If the inspectors are not sure about the concentration or dosage when adding drugs, it will directly cause errors and affe

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  • Tumour cell drug sensitive testing method and its testing plate
  • Tumour cell drug sensitive testing method and its testing plate
  • Tumour cell drug sensitive testing method and its testing plate

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Embodiment Construction

[0057] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0058] There are more than 60 kinds of commonly used anticancer drugs such as doxorubicin (abbreviated as ADM, the same below), cisplatin (COOP), cyclophosphamide (CTX), fluorouracil (5-FU), and vincristine (VCR). , when coating these medicines, carry out according to the following procedure:

[0059] Preparation of anticancer drugs: The drug concentration is calculated according to the conventional intravenous dosage for adults.

[0060] Conventional concentration of anticancer drugs=adult routine intravenous dosage / (adult standard body weight×8%×1000): wherein: adult standard body weight is calculated by 60KG, extracellular fluid is calculated by 8%, and conventional concentration diluted 10 times is the test concentration.

[0061] Drug preparation is carried out according to the relevant instructions of each drug. Taking doxorubicin as an...

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Abstract

A process for detecting the sensitivity of tumor cell to medicine includes such steps as adding the antineoplastic medicine in the holes on detecting plate, freeze solidifying, adding tumor cells to the holes, reducing it to color deposit by MTT method, preparing color solution, enzyme-linked assay, calculation and comparing to obtain sensitivity value, and judging. Its advantages are simple operation, and high correctness and stability.

Description

technical field [0001] The invention relates to a method for testing drug sensitivity of tumor cells, which belongs to the technical field of detection. Background technique [0002] Chemotherapy has an irreplaceable position in cancer treatment that surgery and radiotherapy cannot replace. Chemotherapy has made great progress since Gillman and Phillips reported nitrogen mustards in the treatment of hematopoietic tumors in 1946. Various new anticancer drugs have been continuously developed, and there are more than 60 kinds of anticancer drugs in clinical application. Through unremitting efforts, experts have revealed some of the laws of chemotherapy, and explored a variety of treatment strategies that can help improve the curative effect, such as combined drug use, high-dose intermittent drug delivery, multi-channel multi-modal drug delivery, and dual-channel chemotherapy. At present, chemotherapy has made more than 10 kinds of cancers such as choriocarcinoma, malignant mo...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 崔克勤
Owner 崔克勤
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