Tumour cell drug sensitive testing method and its testing plate

Abstract

A process for detecting the sensitivity of tumor cell to medicine includes such steps as adding the antineoplastic medicine in the holes on detecting plate, freeze solidifying, adding tumor cells to the holes, reducing it to color deposit by MTT method, preparing color solution, enzyme-linked assay, calculation and comparing to obtain sensitivity value, and judging. Its advantages are simple operation, and high correctness and stability.

Description

technical field [0001] The invention relates to a method for testing drug sensitivity of tumor cells, which belongs to the technical field of detection. Background technique [0002] Chemotherapy has an irreplaceable position in cancer treatment that surgery and radiotherapy cannot replace. Chemotherapy has made great progress since Gillman and Phillips reported nitrogen mustards in the treatment of hematopoietic tumors in 1946. Various new anticancer drugs have been continuously developed, and there are more than 60 kinds of anticancer drugs in clinical application. Through unremitting efforts, experts have revealed some of the laws of chemotherapy, and explored a variety of treatment strategies that can help improve the curative effect, such as combined drug use, high-dose intermittent drug delivery, multi-channel multi-modal drug delivery, and dual-channel chemotherapy. At present, chemotherapy has made more than 10 kinds of cancers such as choriocarcinoma, malignant mo...

AI Technical Summary

Problems solved by technology

[0005] 3. Selection of anticancer drugs and avoidance of toxicity. Anticancer drugs kill normal tissue cells as well as tumor cells. Due to the blindness and drug resistance of chemotherapy, when doctors try to use large doses, multiple drugs, and shorten the course of treatment, etc. When the method improves the curative effect, the toxic effect will be further aggravated, causing serious or even fatal damage to the patient's body.

Moreover, due to many steps and a long time, the operation will inevitably be disturbed by human factors. If the inspectors are not sure about the concentration or dosage when adding drugs, it will directly cause errors and affect the colorimetric results, which is very likely to happen. of

In drug susceptibility testing, since the testers generally prepare tumor cells first, and then add the drug to be tested for colorimetric determination, when this operation is carried out in different units, different testers, and at different time periods, there is no international standard for the MTT method. Supported by unified technical standards, the determination of drug concentration, drug and cell reaction time, and the establishment of sensitive standards used by various scientific research institutions and medical departments are different, and such errors are even more unavoidable

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