Bio-chip fluorescent testing scanning device
A biochip and fluorescence detection technology, applied in fluorescence/phosphorescence, emission spectrum, spectrum investigation, etc., can solve the problems of high manufacturing cost, unfavorable miniaturization of the whole machine, and difficulty in achieving high stability and high speed
Inactive Publication Date: 2003-08-13
无锡市朗珈生物医学工程有限公司
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Problems solved by technology
The weak point of this device is: 1, bulky, high power consumption
Due to the use of Ar with extremely low electro-optical conversion efficiency + Lasers have a short lifespan, large volume, and high heat generation, requiring forced cooling, which is not conducive to the miniaturization of the whole machine; and the high manufacturing cost is not conducive to popularization and use; 2. Slow detection speed and low work efficiency
Since one laser is used, only one fluorescent substance image can be obtained; in order to obtain fluorescent images of several fluorescent substances on the biochip, multiple scanning detection devices using various corresponding lasers must be used
Moreover, the device can only detect the fluorescence image of one fluorescent substance on one biochip at a time.
3. Poor detection accuracy
This kind of movement is difficult to achieve high stability and high speed, and the whole device is prone to problems such as misalignment and defocus, thus affecting the accuracy of detection results
Method used
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[0008] Below the present invention will be further described in conjunction with the embodiment in the accompanying drawing:
[0009] The invention mainly consists of a laser excitation optical path, a fluorescence receiving optical path and an X-Y platform on which multiple biological chips can be installed. On the laser excitation optical path, a green solid-state laser 1, a red semiconductor laser 2, a light-combining prism 3, a hollow total reflection mirror 4, and a laser focusing mirror 7 are installed on the bracket 6. A light-combining prism 3 is installed between the green solid-state laser 1 , red-light semiconductor laser 2 and the hollow total reflection mirror 4 , and a hollow total reflection mirror 4 is installed between the light-combination prism 3 and the laser focusing mirror 7 . Along the forward direction of the laser beams emitted by the green solid-state laser 1 and the red semiconductor laser 2, since the light-combining prism 3, the hollow total reflec...
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Abstract
A scanner for fluorescence detection of biochip is composed of laser exciting light path, fluorescence receiving light path, X-Y platform for putting biochip on it, and supporter. After two laser beams are combined by a gathering prism and then come in a same light path, the combined beam passes through the central through holes of a hollow perflector and a focusing lens to be focused to a dot on biochip. The said both light pathes are separated by said perflector and fluorescence splitter. More 2D images of biochip can be obtained by the 2D movement of X-Y platform. Its advantages are compact structure, high scan speed, small size, light weight, low cost, high correctness and low power consumption.
Description
Technical field: [0001] The invention relates to a collection device for fluorescence images of biochips, which is used for scanning detection of fluorescence images on biochips. technical background: [0002] Before the present invention is made, in prior art, the Affymetrix of U.S. Affymetrix company TM Biochip fluorescence detection device (Thane Kreiner published in "American laboratory", March 1996, pages 39-43). The device is mainly composed of Ar + Laser, Shutter, First Turning Mirror, Laser Line Filter, Dichroic Mirror, Second Turning Mirror, Focusing Scan Head, Chip Holder, Laser Intensity Detector, Filter Set, Achromat, Pinhole and Optoelectronics The multiplier tube constitutes the laser excitation optical path and the fluorescence receiving optical path. [0003] Its working process is: Ar + The laser beam emitted by the laser passes through the shutter, the first steering mirror and the laser line filter, and then shoots to the dichroic mirror with monochrom...
Claims
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Inventor 陆善新陈永强
Owner 无锡市朗珈生物医学工程有限公司