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Bamboo forest biological fertilizer

A technology of biological fertilizer and bamboo forest, applied in the direction of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve problems such as pollution

Inactive Publication Date: 2003-09-24
吴晓丽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problems to be solved by the present invention are: reduce or replace the use of nitrogenous fertilizers for bamboo plants, improve the quality of bamboo shoots, adapt to the demand for biological fertilizers during the production of pollution-free green bamboo shoots and organic bamboo shoots, and avoid the flooding of bamboo forests for bamboo shoots and bamboo forests for timber. A kind of bamboo bio-fertilizer is provided by using chemical fertilizers to pollute the environment. This bio-fertilizer can provide nitrogen for bamboo through biological nitrogen fixation. When combined with other ingredients such as phosphorus bacteria, potassium bacteria, organic fertilizers and inorganic fertilizers, it can also improve fertilizer and the utilization rate of phosphorus and potassium in the soil, further improving the soil, improving the micro-ecological environment of microorganisms, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. Prepare solid slant medium for nitrogen-fixing bacteria test tubes, the formula is: 0.5% glucose, 0.5% sucrose, KH 2 PO 4 0.04%, K 2 HPO 4 .3H 2 O 0.01%, MgSO 4 .7H 2 O0.02%, NaCl 0.01%, CaCl 2 .2H 2 O 0.002%, FeCl 3 .3H 2 O 0.001%, Na 2 MoO 4 .2H 2 O 0.0005%, K 2 SO 4 0.15%, yeast extract 0.06%, agar 2%, pH6.8-7.2. After sterilization, respectively insert the four strains of combined nitrogen-fixing bacteria WG-1, WG-2, WG-3 and WG-4 isolated from the rhizosphere of bamboo plants, and cultivate them at 30-32°C to make the surface grow full.

[0069] 2. Prepare a shaker culture medium for nitrogen-fixing bacteria, the formula of which is: 0.5% glucose, 0.5% sucrose, 1% corn flour, K 2 SO 4 0.15%, KH 2 PO 4 0.04%, K 2 HPO 4 .3H 2 O0.01%, NH 4 (SO 4 ) 2 0.1%, MgSO 4 .7H 2 O 0.02%, NaCl 0.01%, CaCl 2 .2H 2 O 0.002%, FeCl 3 .3H 2 O 0.001%, Na 2 MoO 4 .2H 2 O 0.0005%, peptone 0.1%, yeast extract 0.08%, pH6.8-7.2. Put it into a 1000 ...

Embodiment 2

[0077] In Example 1, the production of liquid bio-fertilizer for bamboo forests was carried out by taking 4 strains of combined nitrogen-fixing bacteria isolated from the rhizosphere of bamboo plants as an example. Also can only use any one strain bacterium or any two strains bacterium or any three strains bacterium in the four strains of bacteria in actual production, carry out the production of bamboo forest liquid bio-fertilizer with above-mentioned same method.

Embodiment 3

[0079] 1. Crush the peat, pass through a 80-mesh sieve, dry and sterilize with a 200°C hot air drum, and cool to room temperature.

[0080] 2. Various liquid bio-fertilizers (comprising single strains and mixed strains) made with the method of Example 1 are adsorbed with peat respectively, so that the viable count of functional bacteria reaches the required standard, and the water content<30 % (if the water content is too high, it can be processed by low-temperature air drying) to make various solid bacterial agents containing a single strain or mixed strains.

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PUM

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Abstract

The bamboo forest biolgoical fertilizer capable of meeting the requirements for biological organic fertilizer in production of nuisance-free green bamboo shoots and organic bamboo shoots for reducingor substituting chemical fertilizer is formed from matrix and bamboo plant rhizospheric combined nitrogen-fixing bacteria which are separated from bamboo plant rhizosphere, can be used for fixing molecular nitrogen in atmosphere and are any one strain or combination of any two kinds of strains or more than two kinds of (Bacillus polymyxa mace) WG-1 strain, (Bacillus polymyxa mace) WG-2 strain, (Bacillus licheniformis Chester) WG-3 strain and (Klebsiella pneumoniae (Schroeter) Trevisan) WG-4 strain. Said fertilizer can be used for cultivating bamboo forest.

Description

technical field [0001] The invention relates to fertilizers containing microorganisms, in particular to biological fertilizers for bamboo forests. Background technique [0002] Chemical fertilizers are easy to use, fast in fertilizer efficiency, and can provide essential elements for crop growth, playing a key role in solving the problem of food and clothing for human beings. However, chemical fertilizers also have many disadvantages, such as: a large amount of energy is consumed during production, and the production cost is high; water and air pollution will be generated during production and use, the loss rate is high, and the utilization rate of plants is low; nitrogen fertilizers accumulate in plants after excessive application of nitrogen fertilizers. Nitrite is harmful to human health; due to the single element of chemical fertilizers, it is difficult to match them in a reasonable ratio, which reduces the nutritional value and flavor of agricult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00C05F11/08C05G1/00C05G3/00C12N1/00C12N1/20
Inventor 吴晓丽顾小平
Owner 吴晓丽
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