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Apparatus and method for controlling movement of specimen, and step for processing specimen

A technology for controlling specimens and vibrating devices, applied in methods of sampling biological materials, methods of stress-stimulated microbial growth, post-processing of biomass, etc.

Inactive Publication Date: 2003-09-24
NIHON UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In other words, this operation is performed under a microscope to obtain the desired orientation of a particular specimen, which is very laborious work

Method used

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  • Apparatus and method for controlling movement of specimen, and step for processing specimen
  • Apparatus and method for controlling movement of specimen, and step for processing specimen
  • Apparatus and method for controlling movement of specimen, and step for processing specimen

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Embodiment Construction

[0016] The following describes in detail an embodiment of using a device for controlling specimen movement to control cell movement according to the present invention with reference to the accompanying drawings. Figure 1 shows the standing wave vibration of the vibrator, as well as the result of the flow of the medium and the movement of the specimen, Figure 2 is a schematic diagram showing the structure of the device for controlling the movement of the specimen, Figure 3 is a schematic diagram showing the structure of the processor; A schematic diagram of the voltage waveform.

[0017] First, the principle of controlling the position of the specimen in this embodiment will be described. 1 shows a state where a vibrator 11 (for example, a probe portion 11b) vibrates in a medium 30, and the tip of the vibrator 11 immersed in the medium 30 generates standing wave vibration V, such as vertical vibration. Studies conducted by the inventors of the present application have found th...

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Abstract

A device for controlling the movement of a specimen, thereby processing a specimen, such as a cell, which allows for easier and faster manipulation of the specimen with a high degree of precision. The vibrator (11) is capable of generating standing wave vibration (V) having at least one wave point (N1, N2). The specimen in the medium (30) can be captured at a location near the surface of the wave spots (N1, N2) and then flipped at this location. Through variable control of the standing wave vibration (V) vibration mode, the capture position of the specimen (S) is changed, through variable control of the standing wave vibration (V) vibration parameters (such as frequency, amplitude, intermittent frequency) , you can control the moving route, moving speed, and rotating speed of the desired specimen (S).

Description

technical field [0001] The present invention relates to a technique for controlling the movement of specimens when observing them or performing specified treatments on them, especially for observing small specimens such as cells or performing the aforementioned treatments. Background technique [0002] It is cumbersome to perform various manipulations on such small specimens as cells, which require delicate handling using microtools. An example of performing such a manipulation is microinjection in genetic transformation techniques. In this genetic engineering technique, host cells are pricked with thin glass tubes to introduce recombinant DNA. The microinjection used to introduce recombinant DNA into host cells will be described below. [0003] Introduce host cells as a specimen into a petri dish filled with physiological saline. The operator (worker) uses a suction device to keep the host cells at the top of the suction device, pricks the host cell nucleus with a thin g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L99/00C12M1/42
CPCC12M33/08
Inventor 尾股定夫
Owner NIHON UNIVERSITY
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