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Test strip for chromatography and process for producing the same

A test piece and material technology, applied in the direction of analytical materials, biological material analysis, measuring devices, etc., can solve problems such as not getting correct results

Inactive Publication Date: 2004-08-18
PANASONIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, worry about not getting the correct result

Method used

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  • Test strip for chromatography and process for producing the same
  • Test strip for chromatography and process for producing the same
  • Test strip for chromatography and process for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0035] In this embodiment, a test piece for immunochromatography will be described with reference to the drawings. figure 1 It is a figure which shows the test piece for immunochromatography of this embodiment. figure 2 (a)~ figure 2 (c) is a schematic diagram showing the operation of the immunochromatographic test strip according to the present embodiment.

[0036] like figure 1 As shown, the immunochromatographic test piece 10 of the present embodiment is composed of a base material 11 including a sample introduction part 12 , a labeling part 13 and an immobilization part 14 .

[0037]The substrate 11 is formed of a material impregnated with a solvent of a sample solution containing a substance to be detected, and is formed of a porous material such as nitrocellulose membrane, cellulose acetate membrane, glass fiber filter paper, or nonwoven fabric, for example. Of these, nitrocellulose membranes are particularly desirable. These materials can spread the solvent of t...

Embodiment approach 2

[0063] In the first embodiment described above, the single-layer type immunochromatography test piece was described with reference to the drawings, but in this embodiment, the multilayer type immunochromatography test piece is described. Figure 4 (a) is the figure which shows the upper surface of the test piece for immunochromatography of this embodiment, Figure 4 (b) is along Figure 4 Cross-sectional view of X-X shown in (a).

[0064] like Figure 4 (a) and Figure 4 As shown in (b), the immunochromatographic test piece 40 of this embodiment includes: a substrate 41; a labeling layer 43 and an immobilized layer 44 provided on the substrate 41; The sample introducing layer 42 in contact with the marking layer 43 and the absorbing layer 46 in contact with the immobilization layer 44 are provided on the substrate 41 .

[0065] The material for forming the substrate 41 is not particularly limited, but it is preferable to use a material that does not permeate the solvent of...

Embodiment 1

[0077] In this example, the detection target substance was measured using the test piece 10 for immunochromatography according to Embodiment 1 described above. In addition, the detection target substance in the sample liquid is detected by visually checking the labeling substance 15 accumulated in the immobilization part 14 .

[0078] —Preparation of test strips for immunochromatography—

[0079] Substrate 11 (width 5 mm, length 50 mm) made of nitrocellulose membrane was immersed in 10 mM calcium chloride aqueous solution, taken out as it was, and dried until the water evaporated to form sample introduction part 12 . Then, the anti-CRP serum (immunized animal: goat, manufactured by Cosmobio) was subjected to affinity purification, and the anti-CRP antibody (5 mg / ml) sensitized to the labeled substance colloidal gold ion was used as labeled antibody 15, and 500 μl of the antibody was loaded on a dry The base material 11 forms the marking portion 13 . Then, 200 ml of anti-CRP ...

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Abstract

As shown in FIG. 1, an immunochromatography test strip 10 of the present embodiment is made of a base material 11 which includes a sample introduction section 12, a labeling section 13 and an immobilization section 14. The base material 11 is made of a material that is capable of being impregnated with a solvent of a sample solution containing a detection target substance. The material allows migration of a solvent of a sample solution by capillary action. The sample introduction section 12 is a region where a sample solution containing a detection target substance is introduced (e.g., dripped). The sample introduction section 12 contains a metal salt supported thereon. The labeling section 13 contains a labeled antibody 15, which is labeled with a labeling substance, in a state such that the labeled antibody 15 can be eluted into the sample solution. The labeled antibody 15 used herein specifically binds to a detection target substance. The immobilization section 14 contains an immobilized antibody 16. The immobilized antibody 16 used herein specifically binds to the detection target substance.

Description

technical field [0001] The present invention relates to a chromatographic test piece capable of quickly and accurately quantifying a specific component in a sample liquid in a simple manner. Background technique [0002] Conventionally, there is a dry chemical method as a simple quantification method for a specific component in a sample liquid without diluting or stirring the sample liquid. [0003] The so-called dry chemical method is a method in which a liquid sample is dropped onto a reagent stored in a dry state on a solid substrate such as a film or test paper, and the detection target substance in the sample liquid is measured. [0004] Devices used in dry chemical methods include a single-layer type in which reagents are supported on filter paper, and a multi-layer type in which a developing layer, a reaction layer, a reagent layer, and the like are stacked. No matter what kind of device, because the reagent has been loaded on the solid phase matrix, it does not need...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558
CPCG01N33/558G01N33/54386G01N2333/4737G01N33/54388
Inventor 汤川系子北胁文久河村达朗
Owner PANASONIC CORP
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