Quick-acting diagnosis for mammitis and medicine-allergy test reagent box

Abstract

The invented kit provides features of fast diagnosis and detection, sensitivity, easy of operation without need of professional personnel, and on-site measurement. Bacteriostasis test for extract of Chinese traditional medicine or hydrophobicity medication shows that effect of the invented kit is better than effect of traditional paper strip method. The kit contains reagents of MTT and tetrazole triphenyl chlorinated (TTC). In detecting mastitis of milk cow following steps are carried out: adding milk sample and 0.1ml reagents of MTT and TTC at room temperature for shortest acting time 4-5 minutes; adding soluble color development liquid, and observing result. Based on whether color is appeared, the result is determined.

Description

1. Technical field: [0001] The invention relates to a kit for rapid diagnosis and drug sensitivity test of mastitis, which can be used for rapid detection and diagnosis of dairy cow recessive mastitis, rapid drug sensitivity test of pathogenic bacteria of dairy cow mastitis, bacterial drug sensitivity test, drug antibacterial test and livestock Microbiological testing of poultry products. 2. Background technology: [0002] The incidence of recessive mastitis in dairy cows is 25%-80%, and it is one of the most serious diseases in dairy farms. It can cause diseased milk production to decrease by 20%-30%, thus causing the largest loss, and it is also one of the reasons that seriously affects milk quality and cows are eliminated. Its prevention and treatment mainly rely on the use of antibiotics. For this reason, antibiotics have been abused in recent years. At the same time, antibiotic residues have seriously affected the quality of milk, causing damage to consumers and affect...

AI Technical Summary

Problems solved by technology

However, the above methods can only be used for qualitative diagnosis, and cannot analyze the number of microorganisms in them, let alone perform drug sensitivity tests. At present, it is necessary to first isolate, cultivate and purify bacteria in a professional laboratory, and then conduct drug sensitivity tests to guide clinical accurate medication. Due to the large number of samples required for bacterial isolation, this method is time-consuming and laborious, and cannot be operated on-site. At present, it is only used regularly in a few large-scale farms, and most farms still rely on veterinarians to use medicines based on experience.

So far, there is no medicine kit available for on-site rapid drug susceptibility testing in the Chinese market