Sea Aceta chinensis high value ecolugical utilizing process

An ecological and high-value technology, applied in applications, food preparation, protein food ingredients, etc., can solve problems such as environmental pollution, chitin deacetylation, etc., to reduce pollution, high biological activity, and reduce organic matter. The effect of content

Inactive Publication Date: 2004-12-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of preparing chitin requires the application of strong acid and strong alkali to remove protein and minerals. Strong acid and strong alkali will cause partial deacetylation an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of liquid seeds

[0031] 0.3 parts by weight of beef extract, 1 part of peptone, 2 parts of agar, 0.5 parts of NaCl as the medium, 98 parts of water, pH 7.1, sterilized, streaked with Bacillus subtilis species, cultivated at 30°C for 25 hours , store at 5°C. Transfer the above strains into the eggplant slant (the culture medium is the same as that of the preserved slant), incubate at 30°C for 25 hours, and prepare for use as a bacterial suspension.

[0032] Liquid seed culture. Medium: 3 parts of bean cake flour, 2 parts of corn flour, 1 part of bran, Na 2 HPO 4 0.4 parts, KH 2 PO 4 0.04 parts, 100 parts of water, plus 0.3 parts of soybean oil as defoamer. Sterilize at 120°C for 30 minutes, after cooling, connect 10 parts of the bacterial suspension of the above-mentioned eggplant bottle strains, put in a 150-liter fermenter with a sample volume of 70 liters, at 30°C, ventilate 1:0.6, and stir at 330 rpm , and cultured for 18 hours. At this point...

Embodiment 2

[0043] (1) Liquid seed preparation

[0044] The bacterium suspension of eggplant bottle bacterial classification is the same as embodiment 1.

[0045] Medium: 2.5 parts of bean cake powder, 2.5 parts of corn flour, 1.5 parts of bran, Na 2 HPO 4 0.5 parts, KH 2 PO 4 0.03 parts, 100 parts of water, plus 0.35 parts of soybean oil as defoamer. The amounts of each component are parts by weight. Sterilize at 120°C for 30 minutes, after cooling, connect 8 parts of the bacterial suspension of eggplant bottle strains, put in a 150-liter fermenter with a sample volume of 70 liters, at 31°C, ventilate 1:0.5-0.6, stir 320-330 revolutions per minute and incubated for 17 hours. At this point the pH is 6.3.

[0046] (2) Liquid submerged fermentation of protease preparation

[0047] 1000 liters of fermentation tanks, 450 liters of culture medium are installed, and the composition of culture medium is all parts by weight: 3.5 parts of bean cake powder, 4.5 parts of corn flour, 2.0 part...

Embodiment 3

[0050] Embodiment 3. with embodiment 1, difference is that the preparation of chitin and chitosan is as follows:

[0051] 1 weight part of hairy shrimp enzymatically hydrolyzed and deproteinized residue was soaked in 7 parts of 1M HCl solution at 20° C. for 15 hours, filtered, and the solid residue was washed with deionized water until neutral. Then 0.5M NaOH was added to the product at a ratio of 3:38w / v, and the mixture was placed at 90°C for 1.2 hours. After the reaction was completed, it was filtered, and the precipitate was washed until neutral. The resulting product was chitin, ie chitin.

[0052] Add 12M NaOH to the above-mentioned chitin at 1:2.5w / v and react at 90°C for 2.5 hours to completely deacetylate, then wash the residue until the pH is neutral, freeze-dry to obtain chitosan.

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Abstract

The present invention belongs to the field of applied marine biological technology. The ecological high-value marine shrimp utilizing process includes preparing proteinase preparation with bacillus strain of high proteinase yield and through deep liquid fermentation; enzymolyzing marine shrimp; eliminating dreg; vacuum concentration and spray drying to obtain powdered enzymolyzed short peptide product. The product contains rich free amino acid and functional short peptide, has active peptide content not less than 40%, and has dual nourishing and health functions. The dreg may be used in preparing chitosan with high viscosity. The process realizes the ecological and high value utilization of marine shrimp and has less environmental pollution.

Description

(1) Technical field [0001] The invention relates to a new technical method for high-value and ecological utilization of marine hairy shrimp, and belongs to the technical field of applied marine biology. (2) Background technology [0002] With the increase of the world's population and overfishing, the amount of marine biological resources that can be caught is gradually decreasing. Therefore, the use of biotechnology to optimize the utilization and high-value development of marine biological resources is one of the important research contents for the development of marine high-tech in my country in the future. one. For example, applying enzyme engineering technology to the enzymatic hydrolysis of marine protein resources can produce new food additives and industrial products. The recent development of biotechnology has confirmed that the application of enzyme engineering technology can produce new food and food additives, and can also improve the utilization efficiency of wa...

Claims

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Application Information

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IPC IPC(8): A23J1/04A23L33/18C12P21/00
Inventor 张玉忠何海伦陈秀兰周百成高培基
Owner SHANDONG UNIV
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