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SCAR molecule marker closely linked with wheat anti powdery mildew gene

A powdery mildew resistance gene and a technology for wheat powdery mildew, applied in the field of agricultural biotechnology engineering, can solve problems such as molecular markers that have not been screened out

Inactive Publication Date: 2004-12-22
TIANJIN CITY AGRI BIO TECH RES CENT
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Problems solved by technology

Since the research on molecular markers of wheat powdery mildew resistance genes was carried out in the early 1990s, nearly half of the disease resistance genes have found corresponding molecular markers, but there are still many unknown genes resistant to powdery mildew, and molecular markers have not yet been screened

Method used

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  • SCAR molecule marker closely linked with wheat anti powdery mildew gene
  • SCAR molecule marker closely linked with wheat anti powdery mildew gene
  • SCAR molecule marker closely linked with wheat anti powdery mildew gene

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Embodiment 1

[0046] Using the RAPD molecular marker method, the molecular marker S2092 linked to the wheat powdery mildew resistance gene was obtained 900 .The actual length of the molecular marker is 972bp, which is converted into SCAR by SCAR method 860 、SCAR 200 Two stable molecular markers.

[0047] The specific method is: the parent of the disease-resistant gene donor is the British common wheat variety Brock, which is crossed with the susceptible common wheat line 015 and the susceptible parent Jing 411, and Jing 411 is backcrossed, and the combination is "Brock / 015 / / Jing 4117 ", disease-resistant individual plants are obtained from high-generation lines that are stable against powdery mildew by crossing, backcrossing, and selfing. The resistant and susceptible strains used for linkage identification were Brock / Jing 411F 2 isolated from , including 101 resistant individuals and 30 susceptible individuals;

[0048] 1. DNA extraction

[0049] Take 0.2 g of wheat leaves, grind them...

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Abstract

A molecular marker SCAR associated with the wheat gene resisting powdery mildew features that the RAPD molecular marking method is used to mark the resistances of wheat parents and their hybrid descendant 'Brock / 015 / / Jing411' and screen from them. The 10bp oligonucleoside is used as random primer to obtain new molecular marker S2092 900, which is then transformed to stable SCAR markers: SCAR860 and SCAR200.

Description

technical field [0001] The invention belongs to agricultural biotechnology engineering, in particular to SCAR molecular marker closely linked with powdery mildew resistance gene and its obtaining method. technical background [0002] Wheat powdery mildew caused by the obligate parasitic fungus Erysiphe graminis f.sp.tritici is one of the main diseases that endanger wheat production. In recent years, with the promotion of dwarf and semi-dwarf wheat varieties, the increase of field planting density and the improvement of water and fertilizer conditions, the disease has become more and more serious in the northern and southern wheat regions of my country, and has become one of the main obstacles to high and stable wheat yields. one. In 1990, wheat powdery mildew was prevalent in 39% of the country's wheat growing areas, resulting in an output loss of 400 million kilograms. At present, wheat powdery mildew has become one of the common diseases that threaten wheat production in ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 彭永康陈宏王振英
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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