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New strain APC-20 of Paecilomyces cicadae and fementation process for artificial culture

A technology of APC-20 and Paecilomyces cicadae, applied in the direction of fungi, etc., can solve the problems of growth, no fruiting body, low yield, etc.

Active Publication Date: 2005-01-12
ZHEJIANG CHENGYI PAHRMACEUTICAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural cicadae has become less and less, and the output is low, and it can only be harvested once a year. At the same time, the quality of the product is uneven due to the impact of the environment.
The existing Paecilomyces cicadae in nature has the problems of growth, output, and quality, and cannot adapt to the needs of industrialized production; there are articles to report that natural strains are used to carry out artificial solid culture fermentation and generally do not grow fruiting bodies ( spore bundles); and when used for liquid fermentation, there are problems such as unsuitable technology, low mycelium yield, and uneven quality
This can neither meet the large demand for spore bundles in clinical medicine of traditional Chinese medicine, but also restricts the industrialized deep processing, development and utilization of metabolites of Paecilomyces cicadae

Method used

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  • New strain APC-20 of Paecilomyces cicadae and fementation process for artificial culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: solid culture fermentation method (1)

[0072] Take the APC-20 strain stored in the refrigerator at 4°C and transplant it to the slant culture medium (Note 1) for 7 days, and activate it for use; each 500ml triangular bottle contains 200ml of the first-grade seed medium (Note 2), and insert the slant strain 3 rings of spore powder, placed in a DHZ C oscillator at 25°C, 120r / min, and cultivated for 4 days as first-grade seeds; put 7.5L of secondary medium (Note 3) in a 10L fermenter, sterilize at 121°C for 30 minutes and cool down To 24°C, insert first-grade seeds according to 10% inoculation amount, at 24°C, the tank pressure is about 0.04Mpa, and the air flow is about 0.75m 3 / h, fermented for 48 hours as secondary seeds. After mixing the solid medium (Note 4) and water at a ratio of 1:2, one method can be directly loaded into the culture container, preferably about 2 / 5 of the capacity, placed in a high-pressure steam boiler, heated at 121°C After steri...

Embodiment 2

[0077] Embodiment 2: solid culture fermentation method (2)

[0078] The solid fermentation medium of the present embodiment is bran: corn: bran in a ratio of 2: 1: 1; the solid medium dry material and water are mixed in a ratio of 1: 2.9 weight components;

[0079] The inoculation amount of secondary strains for closed container culture is 3%; the inoculum amount of secondary strains for open culture in curved disc is 10%;

[0080] The culture medium and other processes and steps of this embodiment are the same as those in Example 1.

Embodiment 3

[0081] Embodiment 3: solid culture fermentation method (3)

[0082] Use wheat grains as solid medium, first soak the grains in water overnight, steam until cooked (until the grains crack), and put them into containers or curved discs to occupy one-third of the volume; the secondary or tertiary strains cultivated in closed containers The inoculum amount is 3%; the strain inoculum amount for the open culture of the curved plate is 10%; the rest of the culture process and steps are the same as in Example 1.

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Abstract

In the present invention, a high quality APC-20 bacterial strain (CGMCC, No:1104) is prepared from natural fungal medicine of cicada bloom and a method to produce coremium by fermenting solid of bacterial strain as well as to produce mycelium by fermenting liquid is also disclosed.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to the use of a new strain of Paecilomyces cicadae APC-20 to carry out artificial solid or liquid fermentation to improve the output and quality of spore bundles and mycelia. Background technique [0002] Cicadae has been a precious Chinese herbal medicine with high medicinal value since ancient times. It is a complex parasitized by Paecilomyces cicadae on cicada nymphs. In the past, people mainly collected rare natural cicadae for various health care products or as compatible medicinal materials. But the natural cicadae has become less and less, and the output is low, and it can only be harvested once a year, and the quality of the product is uneven due to the impact of the environment. The existing Paecilomyces cicadae in nature has the problems of growth, output, and quality, and cannot adapt to the needs of industrialized production; there are articles to report ...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 柴一秋陈祝安厉晓腊刘又高王根锷潘玲聪
Owner ZHEJIANG CHENGYI PAHRMACEUTICAL
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