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Method for quick breeding coffee peperomia

A coffee pepper, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult to form large-scale production, in short supply, low natural reproduction coefficient, etc.

Inactive Publication Date: 2005-03-02
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The coffee pepper grass is native to Sri Lanka, and its natural reproduction coefficient is low. It is difficult to form large-scale production by conventional methods (such as cutting with the characteristic of stolon rooting)
At present, China mainly relies on imports, which are expensive and often in short supply

Method used

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Examples

Experimental program
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Embodiment Construction

[0033] A method for rapidly propagating coffee pepper grass, the method is carried out according to the following steps:

[0034] (1) getting the stem tip of the coffee pepper grass with a length of 0.5 to 1.0 cm as the coffee pepper grass culture;

[0035] (2) Rinse the coffee pepper grass culture with running water for 20 to 30 minutes, then soak it with 0.1% mercuric chloride for 6 to 8 minutes, and rinse it with sterile water for 8 to 10 minutes;

[0036] (3) Insert the culture of the coffee pepper grass treated in step (2) into the induction medium, cultivate for 22 to 28 days, and the culture of the coffee pepper grass begins to germinate and grow; the induction medium is: per liter of MS basic medium Add 0.08 mg of KT (6-furanaminopurine), 0.05 mg of 2,4-D (2,4-dichlorophenoxyacetic acid), 30 grams of sucrose, and 6 grams of agar; wherein each liter of MS basic medium contains:

[0037] NH 4 NO 3 (ammonium nitrate) 1650 mg

[0038] KNO 3 (potassium nitrate) 1900mg ...

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Abstract

The present invention discloses the quick breeding method of coffee peperomia. The stem tip of coffee peperomia as the cultured matter is first cultured in the inducing culture medium comprising basic MS culture medium, KT and 2, 4-D, for 3-4 weeks to obtain germinated stem tip, and then cultured in differentiating and proliferating culture medium with BA and NAA to induce the fascicular seedling in the proliferation coefficient of 4-5 times. Then, secondary culture is performed once every 30 days; and over 10,000 seedlings may be cultured in one year. The cultured coffee peperomia is used in aquarium as foreground and medium shot decoration.

Description

Technical field: [0001] The invention relates to a tissue culture method for ornamental aquatic plants, more specifically to a rapid propagation method for coffee pepper grass, which belongs to the application of biotechnology in the production of ornamental aquatic plant seedlings in water cluster boxes. Background technique: [0002] Cryptocoryne petchii belongs to the genus Cryptocoryne petchii in the family Araceae. It is named for its leaf color that can change from green, dark olive to brown depending on the intensity of the light. The leaves of coffee pepper grass are lanceolate and radial, with wrinkled leaf margins, swaying like waves in clear water, very charming, and it is a rare variety in the foreground and middle ground grass of the water cluster box. The coffee pepper grass is native to Sri Lanka and has a low natural reproduction coefficient. It is difficult to form large-scale production by conventional methods (such as cuttings using the characteristic of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘艳玲徐立铭李洪林
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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