Preparation method of tumour immune cell detecting chip and its detecting method

A technology of immune cells and detection chips, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no judgment and early detection of tumor methods, etc., and achieve the effect of high accuracy and convenient use

Inactive Publication Date: 2005-05-25
SOUTHEAST UNIV
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Problems solved by technology

At present, there is no way to judge and detect tumors

Method used

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Embodiment Construction

[0021] The preparation method of the tumor immune cell detection chip of the present invention is as follows: first, carry out chemical modification on the carrier, that is, modify the amino group and aldehyde group, so as to ensure that the chemical gene on the carrier can react with the antibody and be combined on the carrier, and can maintain The immunological activity of the antibody bound to the carrier; then spot the corresponding antibody on the above slides modified with chemical genes, such as: lung cancer, liver cancer, gastric cancer and other tumors, as well as new protein antibodies expressed by gene mutations such as skeletal tumor leukemia, etc. , stored in a humid environment at 0°C-55°C for 0.1-72 hours, then take out the glass slide and wash it with a buffer solution with a pH of 4.0-10 and an ion concentration of 0.00-0.75m / L. The specific steps are:

[0022] 1. Chip production:

[0023] 1).Slide cleaning

[0024] Clean the glass slides with water, put them...

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Abstract

The preparation of tumor immune cell detection chip and detection method relates to a chip for detecting peripheral blood karyocyte of tumor patient and its detection method. The preparation of its chip includes the following steps: on the carrier making chemical modification, namely modifying upper amino-group and aldehyde group; then on the modified microslide on which the chemical gene is set dropping the correspondent antibody, for example, new protein antibody, etc expressed by gene mutation of tumors of carcinoma of lung, live cancer and carcinoma of stomach and skeletal system tumor leucosis, etc. then placing said microslide in humectous environment and storing, then taking out said microslide and washing by using buffer solution. Besides, said invention also provides its detection method by using said chip.

Description

technical field [0001] The invention relates to a method for preparing and detecting an immune chip of nucleated cells in peripheral blood of human tumor patients, and belongs to the technical field of biochip manufacturing and detection. Background technique [0002] Cancer is one of the common diseases that endanger human health. Since the pathogenesis has not been thoroughly explored, there is no effective treatment so far, especially for advanced patients. The prognosis of cancer patients often has a certain relationship with the discovery of cancer. Early detection can be cured by immunotherapy or surgery. If it reaches the middle and late stage, the prognosis of cancer patients is very unfavorable. This shows the importance of early diagnosis of cancer patients. If cancer can be detected and diagnosed early, most patients will be cured. But most of the patients seen clinically are not early cancers. Because early cancer often has no speci...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N33/543G01N33/574
CPCG01N33/54353
Inventor 唐祖明陆祖宏杨玉志张春秀郁颖蕾
Owner SOUTHEAST UNIV
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