Treating solution containing aluminium ion for staining protein and staining method using the same

A technology of staining solution and aluminum ions, which is applied to the preparation method of peptides, chemical instruments and methods, material inspection products, etc.

Inactive Publication Date: 2005-07-20
姜撤勋
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Requires about 1 to 2 days to stain and decolorize proteins, a method with a relatively long processing time

Method used

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  • Treating solution containing aluminium ion for staining protein and staining method using the same
  • Treating solution containing aluminium ion for staining protein and staining method using the same
  • Treating solution containing aluminium ion for staining protein and staining method using the same

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Embodiment

[0045] Use 7×10cm sodium dodecyl sulfate-polyacrylamide gel to separate rabbit skeletal muscle myosin, E.coli phosphorylase b, rabbit skeletal muscle galactosidase, bovine serum albumin, egg white egg white After electrophoresis of purified proteins consisting of protein and bovine carbonic anhydrase, the gels removed from the glass plates were washed briefly with deionized water. The gel was treated three times for 30 minutes with a first solution (50 ml) containing 30% ethanol and 2% phosphoric acid in water, and three times for 30 minutes with a second solution (50 ml) containing 2% phosphoric acid in water.

[0046]Then, the gel was soaked for 30 minutes in a third solution (50 ml) comprising 15% aluminum sulfate, 20% ethanol and 2% phosphate solution. Then, a fourth solution containing 2% G250 and 0.2 g / L sodium azide was added thereto to have a final concentration of 1% of the total solution (the final concentration of CBB G250 was 0.002%). Here, proteins were subjected...

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Abstract

The present invention generally relates to treatment solutions for staining proteins including aluminum ions and staining methods using the same, and more specifically, to a treatment solution for staining proteins including aluminum ions, the treatment solution used when proteins are stained by a Commassie Brilliant Blue CBB stain, and a staining method using the same. The disclosed treatment solution including aluminum ions for CBB-staining proteins can decrease detection limits, thereby improving the sensitivity and reducing staining time as well as omitting a de-staining process.

Description

technical field [0001] The present invention relates to a staining solution for protein staining containing aluminum ions and a staining method using the same. Background technique [0002] Protein staining, which is important for the analysis of experimental results in proteomics, is the step of making proteins in two-dimensional space visible. Hereinafter, "dying" described in the specification means "staining". [0003] Two-dimensional gel electrophoresis (hereinafter referred to as "2-D gel electrophoresis") is a method of unfolding proteins in cells in a two-dimensional space using differences in molecular weight and electric charge that are characteristic of proteins. The comparative analysis of protein expression differences under various conditions was performed on the basis of 2-D gel electrophoresis. [0004] Traditionally, protein staining based on 2-D gel electrophoresis in proteomics is performed by staining techniques such as silver staining, fluorescence-bas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/13G01N33/68
CPCC07K1/13G01N33/6839G01N33/683
Inventor 姜撤勋徐命九
Owner 姜撤勋
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