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Correlation between ornithine decarboxylase 1 gene and primary hypertension

A technology of ornithine decarboxylase and gene, applied in the field of molecular biology and medicine, can solve the unconfirmed report of the correlation between ODC1 gene and essential hypertension, and the unconfirmed correlation between ODC1 gene SNP and essential hypertension Sexual reporting and other issues

Inactive Publication Date: 2005-08-17
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although there have been many studies on the relationship between various gene polymorphisms and essential hypertension, there is no report on the correlation between the ODC1 gene and essential hypertension, let alone the correlation between the SNP of the ODC1 gene and essential hypertension sex reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1.1 Research object

[0066]Since blood pressure is a quantitative trait that is affected by multiple genes and is affected by various environmental factors, it is necessary to consider the issue of genetic heterogeneity when analyzing related genes. Selecting isolated populations with a relatively single genetic background as materials for the study of polygenic diseases will help reduce the impact of genetic heterogeneity. reduce. Selecting such a group, whether it is used for linkage disequilibrium analysis or selecting families for family analysis, will help to improve the detection sensitivity of hypertension susceptibility loci. Therefore, in this embodiment, the isolated population is selected as the research object (the isolated population has the advantages of relative consistency of genetic background and relatively small number of founders).

[0067] On the basis of informed consent, 324 individuals of Han nationality over the age of 50 were randomly collec...

Embodiment 2

[0086] Essential Hypertension Susceptibility Detection Kit

[0087] As described in Example 1, the G→A mutation at position 4823 of SEQ ID NO: 1 is closely related to hypertension. Therefore, based on this mutation, ODC1 gene-specific primers can be designed to amplify with the patient's DNA as a template for detection.

[0088] name

sequence

concentration

sense primer

SEQ ID NO: 2

100pmol

antisense primer

SEQ ID NO: 3

100pmol

PCR reaction solution

Containing Taq Enzyme dNTP Magnesium Ion PCR Reaction Buffer

[0089] Take 3ml of blood from the male patient to be tested, and use a conventional method (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the high blood pressure detection kit to 1μmol / μl, and use the extracted DNA as a template to perform a PCR reaction with the provided primers. After purification of PCR products, ABI-PRISM TM 377 DNA sequencer was used for two-way seq...

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Abstract

The present invention discloses method of detecting primary hypertension susceptibility. The detection includes detecting individual ornithine decarboxylase 1 gene ODC1, transcript and / or protein, comparing with normal items to find the existing variation, which shows the greater attack probability of the individual in primary hypertension. The present invention also discloses the corresponding detection kit.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (single nucleotide polymorphism, SNP) of ornithine decarboxylase 1 gene (ornithine decarboxylase 1, ODC1) and its correlation with essential hypertension. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Hypertension refers to a group of clinical syndromes with elevated systolic or diastolic blood pressure. There is an obvious causal relationship between the increase of blood pressure and the occurrence of coronary heart disease, renal dysfunction, hypertensive heart disease and hypertension complicated with stroke. The latest diagnostic criteria for hypertension are systolic blood pressure ≥ 19kpa (140mmHg) or diastolic blood pressure ≥ 12kpa (90mmHg), and those who meet one of them can be diagnosed as hypertension. [0003] Most hypertensive ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
Inventor 金力黄薇姜正文王颖张晨辉李艳平王志敏肖君华卢大儒
Owner FUDAN UNIV
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