Primer and probe for detecting vibrio vulnificus and detection method using the same

Abstract

To produce a high-performance specific gene amplification primer for detecting, quantifying, or identifying Vibrio vulnificus, having low risk of misidentification and practically sufficient amplification efficiency and amplification specificity. We have determined partial nucleotide sequences of gyrB, rpoD, and recA genes of Vibrio vulnificus and the closely related species, revealed their phylogenetic relationship, and then identified nucleotides characteristic to Vibrio vulnificus. Thus, we have made it possible to design a probe having high specificity and a gene amplification primer having high specificity and excellent amplification efficiency, both of which contain the characteristic nucleotides.

Description

technical field [0001] The invention relates to a method for detecting, identifying, or quantifying Vibrio vulnificus in food detection, epidemiological environment detection, and clinical inspection. Background technique [0002] Vibrio vulnificus is a halophilic Gram-negative bacterium found in seawater and brackish water areas. Although rarely infecting healthy humans, Vibrio vulnificus infects people with serious primary diseases such as liver disease. cirrhosis of patients and can cause severe infectious disease. As an infectious bacterium associated with fatal cases, it has attracted attention in public health. The first case reports of infectious diseases produced by this bacterium include by Roldan in 1970 ( New Engl.J.Med., 282,1306,1970) isolated bacteria from lower gangrene. This case was initially considered to be a case of extraintestinal infection of Vibrio rahaemolyticus. Since then, it has been revealed that this bacterium degrades lactose more than large int...

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Problems solved by technology

Furthermore, based on the primers designed, no comparison was made to these hemolysin genes

Therefore, it cannot be said that specificity has been sufficiently demonstrated

[0007] As mentioned above, it cannot be said that the specificity is sufficient with regard to the existing methods for detecting Vibrio vulnificus using genes

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