Natural calculus bovis in vitro breeding method

A technology of natural bezoar and cultivation method, applied in the field of in vitro cultivation of natural bezoar, can solve the problems of inability to realize large-scale production, inability to industrialize development, low formation of ox gallstones, etc., and achieve stable and controllable quality, complete functions, and wide effects. Effect

Inactive Publication Date: 2005-10-26
苟仕金
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Problems solved by technology

Looking at these achievements and patents, they can be summarized into five categories: one is induction in the gallbladder of cattle by surgery; the other is induction by embedding a culture bottle in the body of a cow; Although some of these technologies apply for patents, they cannot be developed industrially because of the low formation of ox gallstones; although some have declared the production batch number, they cannot realize large-scale production; The bovine gallstone method has truly realized the industrialized production and the purpose of culturing gallstones in vitro
However, at present, Escherichia coli, Enterococcus, and Proteus are mostly used to prepare natural bezoar fermenting microorganisms, and a special bezoar cultivation machine is required, which is protected by patents. Most of the products contain pyrogens, and the products produced cannot be used for intravenous injection. The raw materials and process are relatively complicated, and the product quality is still unstable

Method used

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  • Natural calculus bovis in vitro breeding method

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example 1

[0021] Example 1, natural bezoar in vitro cultivation method

[0022] (1) Collect fresh ox gall

[0023] Collect the gallbladder of fresh cattle after slaughter in the cattle slaughterhouse, take out the bile, put it in a container and seal it, and store it under the condition of 2-4°C and avoid light

[0024] (2) Form Calculus Calculus active ingredient (see Table 1)

[0025] (3) The active ingredients of bezoar are added to ox bile to form a mixed solution

[0026] Take an appropriate amount of ox bile, add the active ingredients of bezoar, measure the content of taurine, adjust the content of taurine to 6-10% with bile, and store it at 2-4°C;

[0027] (4) Preparation of effective compound microbial preparations

[0028] Effective compound microbial bacterial liquid 0.8-1.2, distilled water 48-52, glucose 2-3, sodium chloride 0.4-0.6, thiamine 0.8-1.2; mix the above substances fully, put them into a container and cover, not necessarily airtight, put Bacteria are bred and...

example 2

[0040] Example 2, the nutritional formula of effective microorganisms for the preparation of natural bezoar: 1 kg of effective compound microbial bacterial liquid, 50 kg of distilled water, 2.5 kg of glucose, 0.5 kg of sodium chloride, and 1 kg of thiamine.

example 3

[0041] Example 3, mixed fermentation of effective microorganisms and ox bile: take 8000-12000 milliliters of mixed liquid in parts by weight, and 400-600 milliliters of effective compound microbial preparation bacterial liquid, and put them into fermenters in proportion for fermentation; place them at 22-26 ° C for 3-5 Ferment for 1 day, and stir once every 4-8 hours, each time for 25-35 minutes, when the pH value of the fermented product reaches 3-4, it is moderate, take it out and pack it, and store it at 2-8°C for later use.

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Abstract

A process for externally culturing the natural ox gallstone includes collecting fresh ox gall, preparing the active components of gallstone, adding them collected ox gall, adding the active microbe composition, fermenting, preparing saturated solution of calcium oxide, preparing composite cholerythrin calcium, preparing ox gallstone, and examining quality.

Description

[0001] Field [0002] The invention relates to the technical field of traditional Chinese medicine and pharmacy, in particular to a natural bezoar in vitro cultivation method in which bovine bezoar is added to bovine bile, fermented with effective compound microorganisms, and placed in a low-speed rotating container. Background technique [0003] Calculus bezoar is dried bovine gallstones and is a valuable traditional Chinese medicinal material. Because the natural stone formation rate in cattle is extremely low, the sources of bezoar medicine are scarce, rely on imports, and are expensive. At present, there are 650 kinds of patent medicines containing bezoar in China, and 42 kinds of Chinese patent medicines are forbidden to use artificial bezoar. Therefore, the demand for natural bezoar is large, and the existing production is far from meeting the needs of the development of my country's national pharmaceutical industry. In order to change the shortage of natural bezoar dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413
Inventor 苟仕金苟鸿鹰
Owner 苟仕金
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