Method for extracting compound of carotenoid and flavonoid from leaves of purple operilla
A technology of flavonoids and carotene, which is applied in the production of organic chemistry and bulk chemicals, can solve the problems of high price of fresh leaves, small yield, and ineffective use of natural nutrients, and achieve simple production process and easy The effect of operational control
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Embodiment 1
[0009] Example 1: Dry the harvested perilla leaves naturally in the shade or at 30°C, coarsely crush them to 1 mm, and use supercritical CO 2 Extraction, the extraction temperature is 20°C, the extraction pressure is 10Mpa, the extraction time is 5h, and the oily extract and residue are obtained, wherein the oily extract is the extracted carotenoid (content is 300mg / kg), and the concentration is 40% The ethanol extracts the residue after drying or directly dry perilla leaves, the solid-to-liquid ratio is 1:10, the extraction temperature is 30°C, the extraction time is 5h, and the extraction is performed 3 times, and the ethanol extracts are combined, The paste extract obtained after distilling and recovering ethanol is the extracted flavonoids (content is 40mg / g).
Embodiment 2
[0010] Example 2: Dry the harvested perilla leaves at a temperature of 60°C at a low temperature, coarsely crush them to 10 mm, and use supercritical CO 2 Extract, and use perilla oil as the entrainer of extraction, its consumption is by weight: perilla leaf: perilla oil=30: 1 (W / W), and extraction temperature is 45 ℃, and extraction pressure is 40Mpa, and extraction time is 1h, to obtain the oily extract and residue, wherein the oily extract is the extracted carotenoid (content is 1000mg / kg), continue to use 95% ethanol to soak the dried residue or directly dry perilla leaf Extraction, the solid-liquid ratio is 1:20, the extraction temperature is 70°C, the extraction time is 1h, the extraction is performed 5 times, the ethanol extraction solution is combined, and the paste extract obtained after distillation and recovery of ethanol is the extracted flavonoids compound (content is 10mg / g).
[0011] The carotenoids and flavonoids obtained in the above examples can be made into...
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