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Method for preparing wool keratinase

A technology of keratinase and protease, which is applied in the field of preparation of wool keratinase, can solve the problems of increasing the dosage and acting time, affecting the strength of woolen sweaters, and the effect is not ideal, and achieves the effect of being convenient for promotion.

Inactive Publication Date: 2005-10-26
浙江雀屏纺织化工有限公司
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Problems solved by technology

However, since the enzymes produced by enzyme preparation manufacturers mainly refer to proteases, but not for wool scales, the effect of this enzyme on wool scales is not ideal. The weight reduction rate is 6~10%, but the effect on descaling is not ideal, and the effect of chlorinated descaling cannot be achieved, and the main layer of wool fiber - the cortex layer is severely damaged, affecting woolen sweaters Strength of

Method used

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  • Method for preparing wool keratinase

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Embodiment 1

[0017] Embodiment 1: wool keratinase preparation

[0018] Protease-producing bacteria ——→ slant culture, add 0.5% wool powder to the culture medium——→ pick out high-incidence strains after culture——→ fermentation culture medium: soybean flour 5%, corn flour 1.0%, fish meal 1.0%, , CaCl 20.5%, NH4CL 0.5%, Na2HPO4 1.0%, wool powder 0.5%, and the rest water, at a temperature of 30°C and a shaker speed of 200 rpm, shake culture for 88 hours——→Fermentation broth coarse filtration—— → filtrate separation, at 3000 rev / min, 10 minutes——→ obtain wool keratinase liquid.

[0019] The wool keratinase prepared according to the above method is used for descaling and post-finishing, such as taking a woolen sweater sample --→ pre-treatment is 30% concentration of H 2 o 2 30ml / L, Na 2 CO 3 0.2%, Na 2 SO 3 0.7%, JFC 3%, bath ratio 1:25, treatment at 50°C for 35-40 minutes—→add H2O2 enzyme to remove excess H2O2—→water washing—→dehydration—→enzyme peeling treatment, that is, PH=3.0 ~4.0...

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Abstract

This invention relates to a making method of wool keratinase. One step is after bevel cultivate to proteinase production bacterium, add wool powder 0.5% to culture medium. Two step is to select bacterium strain, ferment cultivate, Three step filtrate ferment liquid, separate filtrate liquid, get wool keratinase liquid, The percent of cultivate medium in ferment cultivate as follows, soybean powder 5%, corn powder 1.0%, fish powder 1.0%, CaCl2 0.5%, NH4Cl 0.5%, Na2HPO4 1.0%, wool powder 0.5%, the other is water, the circumgyrate is 200 circles per minute, oscillation cultivate needs 88 hours.

Description

technical field [0001] The invention relates to a preparation method of wool keratinase. Background technique [0002] The finishing of wool fabrics, such as: anti-shrinkage, softness, mercerizing, etc., is the last processing link in the production of wool fabrics. Through this link, the wool fabric can obtain excellent clothing performance and greatly increase its added value. [0003] Wool is a natural protein fiber with a layer of scales on its surface, which overlap and cover the hair shaft like fish scales or tiles. Due to the different friction coefficients in the forward and reverse direction of the scales, when the woolen sweater is worn, under the repeated action of external force, the wool fibers will interpenetrate, entangle, and feel together to produce fluffing; at the same time, the existence of the scale surface is not conducive to softness and mercerization. tidy. Therefore, in the finishing process of woolen sweaters, the first ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50
Inventor 杨豪杰姚恕倪利强
Owner 浙江雀屏纺织化工有限公司
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