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Seedlings raising method for non-shell monospore cell of laver

A single spore and shell technology, which is applied in the field of laver shellless single spore cell seedling cultivation, can solve the problems of low seedling raising efficiency, time-consuming, labor-intensive costs, poor stability, etc., and achieve high seedling raising efficiency, simple and easy operation, and huge social and economic benefits Effect

Inactive Publication Date: 2005-11-02
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than 99% of the seaweed seedlings in Sanguo adopt the traditional shell filament seedling technology. Each hectare (equivalent to 15 mu) of seaweed net curtain needs at least 15 square meters of indoor pools to cultivate shell filaments. The seedling period is as long as 5 ~10 months, there are long-term problems such as low seedling efficiency, poor stability, time-consuming, labor-intensive and high cost to be solved by research
Monospores are originally the reproductive cells of Porphyra, which have great potential for application and development. Although phycologists and producers have known and understood monospores for more than 50 years, they have not been researched and developed into a satisfactory productive monospore. Spore breeding technology system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The mother seedling net is derived from Porphyra zebra strain 0106 (wild type), which can release a large number of monospores. First, the long-term preserved suspension filamentous cells of the 0106 strain were inoculated on the shells on April 20 using traditional seedling cultivation techniques. For a single strain of shell filaments, on September 20, a specific indoor collection of conchospore seedlings was carried out on the Internet. After the seedlings were collected, these batches of conchospore seedlings were immediately transplanted to the sea area for sea emergence. By October 15, they had been in the sea The sea develops and grows into a mother seedling net that releases a large number of monospores. Take it back from the open sea, put it in a closed land tank to collect monospore seedlings on the net, and implement effective tracking, counting and monitoring of the single spore release process. To determine, the inoculation density of this embodiment is to ...

Embodiment 2

[0015] The difference from Example 1 is:

[0016] The mother seedling nets are derived from the high-quality seedling nets of P. On October 24th of that year, one of the high-quality seedling nets (with an area of ​​10m 2 ), stored in a freezer at minus 18°C. On October 6 of the next year (the storage period is 374 days), take it out as a mother seedling net, put it in a 20°C seawater tank for recovery treatment for 2 days, and put the net back into the 20°C water tank after drying in the morning on October 8 , the inoculation density is to inoculate 100 million single spores per mu of blank seedling nets, and a large number of single spores will be released in only 10 minutes (including 6 minutes of swinging), and the release will be completed in 30 minutes. Immediately put into the blank net to pick seedlings. The amount of seawater per mu of mother seedling net is 0.5 tons; the dispersal and inoculation of single spores in the water tank were repeated 3 times on the same...

Embodiment 3

[0019] The difference with embodiment 1 and 2 is:

[0020] The mother seedling net comes from the high-quality mother seedling net of the Porphyra zebra 0312 strain bred by the indoor test of suspended filamentous seedlings, and is releasing a large number of monospores. Choose one of the high-quality seedling nets (area 2m 2 ), put it in a freezer at minus 24°C for 3 months, took it out as a mother seedling net, recovered it under the artificial control temperature of 18°C ​​for only one day, then put it in the water tank to start releasing monospores, and inoculated the seedling net. The inoculation density is 300 million spores, the seawater temperature in the tank is 18°C, the single spore release time is 30 minutes (including 6 minutes of swinging), and the seawater consumption per mu of mother seedling net is 0.7 tons; the mother seedling net is taken out after each release Put it in the air, and immediately take the water samples in the tank to count, then collect the ...

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Abstract

The no-shell monosporous cell laver seedling growing process features the setting the collected monosporous laver seedling on net inside closed water tank through spreading monospores, tracing and counting, monitoring and controlling the amount of monospores in 0.1-0.3 billion each mou of curtain. The mother seedling net capable of spreading great amount of monospores may be obtained through traditional shell filament seedling growing process in the same year or sorted, freeze maintained and recovered monosporous seedling grown within 18 months. Compared with traditional complicated laver seedling growing process, the present invention has simple, feasible, reliable and stable.

Description

technical field [0001] The invention relates to seaweed cultivation, in particular to a method for cultivating laver shellless monospore cells. Background technique [0002] Laver is the most important artificially cultivated seaweed, and its output value accounts for two-thirds of all artificially cultivated seaweed in the world. At present, only three countries, China, Japan and South Korea, have the industry of artificially cultivated seaweed. More than 99% of the seaweed seedlings in Sanguo adopt the traditional shell filament seedling technology. Each hectare (equivalent to 15 mu) of seaweed net curtain needs at least 15 square meters of indoor pools to cultivate shell filaments. The seedling period is as long as 5 ~10 months, long-term problems such as low seedling efficiency, poor stability, time-consuming, laborious and high cost need to be studied and solved. Finding more advanced, stable and reliable new technology of shellless filamentous phytophthora seedlings h...

Claims

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Application Information

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IPC IPC(8): A01G33/02
Inventor 费修绠于义德梅俊学张京浦彭光邹立红李世英陈兰涛汤晓荣姜鹏
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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