Anti-tag antibody chip for protein interaction analysis

A technology for labeling antibodies and proteins, which can be used in the analysis of materials, material analysis by optical means, and analysis by chemical reaction of materials, etc., and can solve the problem of unable to maintain protein structure and function.

Inactive Publication Date: 2005-11-16
HITACHI HIGH-TECH CORP
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Problems solved by technology

[0009] Protein chips are expected to be used as a tool for functional analysis even in proteomics analysis, but when proteins are immobilized on a support such as a glass slide directly or using a protein immobilization reagent, the structure and function of the protein cannot be maintained more often

Method used

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  • Anti-tag antibody chip for protein interaction analysis
  • Anti-tag antibody chip for protein interaction analysis
  • Anti-tag antibody chip for protein interaction analysis

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Embodiment Construction

[0023] The above and other novel features and effects of the present invention will be described below with reference to the accompanying drawings. However, the drawings are exclusively for illustration and do not limit the scope of the present invention.

[0024] 1 is a schematic structural view of an anti-label antibody chip according to an embodiment of the present invention, which consists of chip 1, a partial enlarged view of chip 1, and a diagram showing the binding state of chip 1 and target fusion target protein 5 via anti-label antibody 6.

[0025] There are m×n dots 2 arranged vertically and laterally on the chip 1 . The chip 1 is composed of a support 3 and a layer of a protein-immobilizing reagent 4 , which binds to a label-fusion target protein 5 via an anti-label antibody 6 , whose surface is treated. The tag-fused target protein 5 is composed of the target protein 8 and the tag 7, and binds to the anti-tag antibody 6 through the tag 7 part. The target protein ...

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Abstract

PROBLEM TO BE SOLVED: To provide an anti-tag antibody chip which enables the execution of the functional analysis of protein on a solid phase with high density and high throughput regardless of the kind of protein.

Description

technical field [0001] The present invention relates to an anti-label antibody chip for performing functional analysis of proteins on a stationary phase in a high-density and high-throughput manner, and related technologies. Background technique [0002] With the progress of genome analysis of various biological species, the accumulation of a large amount of base sequence information is forming a world scale. In living organisms, proteins are synthesized through transcription and translation processes based on these nucleotide sequence information, and various activities of living organisms are maintained through this function. With the enrichment of genome information, in recent years, analysis of the structure and function of proteins that play the role of functional molecules in living organisms has become popular, which is called proteomics or proteonome analysis. [0003] Examples of protein functional analysis in liquid phase include enzyme activity assays and inhibit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K1/22C12N15/09G01N21/27G01N21/78G01N33/00G01N33/543G01N33/58G01N37/00
CPCG01N33/54373
Inventor 神田胜弘笹仓由贵江
Owner HITACHI HIGH-TECH CORP
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