Ossotide injection and preparation method thereof

A peptide injection, osteopeptide technology, applied in bone diseases, pharmaceutical formulations, peptide/protein components, etc., can solve the problems of complex process of osteopeptide injection, low content of active ingredient osteopeptide, and limited effective application, etc. The effect of large-scale industrial production, high content of osteopeptide and less impurities

Inactive Publication Date: 2005-11-23
赵红梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional preparation method of osteopeptide injection has defects such as complex process and cumbersome steps (referring to "Animal Biochemical Pharmacy" P283, published b

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take fresh pig limb bones, wash and crush them, weigh 50kg, add 100L 0.15mol NaCl solution, extract at 0.12MPa, 123℃ for 2h, centrifuge and filter with 100-mesh fine cloth, extract the bone residue again under the same conditions and filter, combine Take the filtrate twice to obtain 200L of the extract; put the water extract at room temperature first, then transfer it to a refrigerator for overnight storage at 4°C, remove the upper layer of fat the next day, warm the lower part of the extract, and then centrifuge it with 100-mesh fine cloth; filter Put the liquid into a vacuum concentration tank, when it is concentrated to 35L, let it out to cool down, add 70L of 95% ethanol under stirring, stir for 10min, let it stand still, after the precipitation is complete, siphon out the supernatant alcohol liquid; The medicinal solution is about 34L; add 34L distilled water to the aqueous medicinal solution, stir well, and then pass through a weakly acidic cationic resin column (7...

Embodiment 2

[0019] 1. Take the frozen and fresh-preserved sheep limb bones, after thawing, wash and crush them, weigh 100kg, add 200L of 0.2mol NaCl water, extract by hot pressing at 0.122MPa, 125°C for 1.5h, and use a 100-mesh filter cloth Centrifuge. The bone dregs were extracted again under the same conditions, filtered by rejection, and the two filtrates were combined to obtain 400 L of extract.

[0020] 2. After the water extract was cooled to room temperature, it was transferred to a refrigerator and stored overnight at -4°C. The next day, the upper layer of fat was removed, and the lower part of the extract was slightly warmed, then centrifugally filtered with a 100-mesh filter cloth.

[0021] 3. Put the filtrate into a vacuum concentration tank, and when it is concentrated to 70L, release the liquid medicine to cool down, add 150L of 95% ethanol under stirring, stir for 10min, let stand, and after the precipitation is complete, siphon out the supernatant alcohol liquid.

[0022]...

Embodiment 3

[0028] 1. Take the frozen and fresh-preserved bovine limb bones. After thawing, wash and crush them, weigh 100kg, add 200L of 0.18mol NaCl water, extract by hot pressing at 0.122MPa, 125°C for 1.5h, and use a 100-mesh filter cloth Centrifuge. The bone dregs were extracted again under the same conditions, filtered by rejection, and the two filtrates were combined to obtain 400 L of extract.

[0029] 2. After the water extract was cooled to room temperature, it was transferred to a refrigerator and stored overnight at 8°C. The next day, the upper layer of fat was removed, and the lower part of the extract was slightly warmed, then centrifugally filtered with a 100-mesh filter cloth.

[0030] 3. Put the filtrate into a vacuum concentration tank, and when it is concentrated to 70L, release the medicinal liquid to cool down, add 200L of 95% ethanol under stirring, stir for 10min, let stand, and after the precipitation is complete, siphon out the supernatant alcohol.

[0031] 4. P...

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PUM

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Abstract

Disclosed is an ossotide injection and preparation method, wherein the preparation comprises the following steps, (1) extracting from the bone of mammals through salt solution heat pressing and boiling, centrifugal filtrating, merging the extract, dispensing 1-40 hours at low-temperature, subjecting the raffinate to centrifugal filtration, collecting the filtrate for later use, (2) vacuum concentrating the filter liquor and charging ethanol, stewing and settling, filtering the upper layer alcohol liquid, reclaiming ethanol, obtaining water extract, (3) charging distilled water of the equal volume, passing in turn through weakly-acidic cationic resin column, weakly-basic anionic resin column, pearl form chitosan column and chitosan modified activated charcoal column, rouging and purifying, obtaining pure thin soup, (4) concentrating the pure thin soup, passing through microporous filtration film under pressurization, loading the filtrate, sealing and hot-pressed sterilizing, or freezing and drying the obtained soup, thus obtaining freeze-dried powder injection.

Description

technical field [0001] The invention relates to a preparation method of an injection, in particular to a preparation method of an osteopeptide injection and a product obtained by the preparation method, and belongs to the field of medicines. Background technique [0002] Bone peptide injection (Gu Ning injection) is extracted from the limb bones of mammals such as pigs or cattle. Since it was developed by Nanjing Biochemical Pharmaceutical Factory in 1976, it has been used clinically for nearly 30 years, mainly for the treatment of various joints Inflammation, can reduce inflammation and relieve pain, and the curative effect is definite. In recent years, the therapeutic scope of the found osteopeptide injection has become wider and wider, such as for the treatment of osteoporosis (Liu Weirun et al., Journal of Binzhou Medical College, Vol. 26, No. 6, 2003, pp395), and also for the treatment of Fracture nonunion of long bones of extremities (Xue Yunfeng, Henan Journal of Sur...

Claims

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Application Information

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IPC IPC(8): A61K35/32
Inventor 赵红梅
Owner 赵红梅
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