Subtilases

A technology of subtilase and JP170, which is applied in the field of modifying subtilase using protein modeling based on the three-dimensional structure of JP170, can solve the problem that the three-dimensional structure of subtilase is not disclosed.
CN1791672AInactive Publication Date: 2006-06-21NOVOZYMES AS

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NOVOZYMES AS
Publication Date
2006-06-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method for non-targeted complex sample analysis which involves the following steps. A first step involves providing a database (16) containing identifying data of known molecules. A second step involves introducing a complex sample containing multiple unidentified molecules into a Fourier Transform Ion Cyclotron Mass Spectrometer (12) to obtain data regarding the molecules in the complex sample. A third step involves comparing the collected data regarding the molecules in the complex sample with the identifying data of known molecules in order to arrive at an identification through comparison of the molecules in the sample.
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Description

technical field

[0001] The present invention relates to JP170 and BPN'-like subtilases and methods of constructing these variants with altered properties, such as stability (e.g. thermostability or storage stability), Ca 2+ Dependent, pH-dependent activity for better performance in washing and cleaning applications. Background technique

[0002] Enzymes have been used in the detergent industry for over 30 years as part of washing formulations. Proteases are the most relevant enzymes in these formulations from a commercial point of view, but other enzymes including lipases, amylases, cellulases or enzyme mixtures are also frequently used.

[0003] To improve the cost and / or performance of proteases, proteases with altered properties are being sought such as: higher low temperature activity, higher thermostability, higher specific activity at a given pH, altered Ca 2+ dependence, greater stability in the presence of other detergent ingredients (eg bleach, surfactants, etc.)....

Claims

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