Cynoglossus semilaevis gunther specific molecular label and genetic sex identifying method

A kind of semi-smooth tongue sole and molecular marker technology, applied in the field of fish sex-specific molecular marker and genetic sex identification

Inactive Publication Date: 2006-08-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

There are no reports at home and abroad about sex-specific molecular m

Method used

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Embodiment Construction

[0023] Using modern molecular biology techniques to screen out sex-specific molecular markers and establish molecular techniques for genetic sex identification of tongue sole, which will help to cultivate all-female seedlings of tongue-sole half-smooth sole, carry out breeding of all-female seedlings, increase the breeding yield of tongue-sole half-smooth sole, and improve the economy of breeding It is of great practical significance and huge economic benefits to really develop tongue sole into an excellent cultured species accepted by the majority of farmers, promote the development of tongue sole aquaculture industry and the replacement of marine fish culture species.

[0024] Below, the technical content of the present invention will be described in detail through the screening of female-specific AFLP molecular markers of half-smooth tongue sole and the establishment of genetic sex identification method:

[0025] 1. Sex-specific molecular markers of tongue sole, including: 1...

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Abstract

This invention relates to specific molecule labeling of the sex of semi-sliding tongue soles and its genetic sex test method including the pick-up of the blood DNA, the AFLP analysis of DNA in the gene set, selection of the AFLP molecular labeling and a method for genetic sex check up of semi-sliding tongue sole, which selects 4 from 64 primer combinations to generate female specific DNA segments and selects 7 female specific AFLP molecular labels of the sole to set up the molecule labeling technology of its genetic sex authentication.

Description

Technical field: [0001] The invention belongs to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, and is a fish sex-specific molecular marker and genetic sex identification method that can be applied in fish parthenogenesis. Background technique: [0002] my country is rich in fish resources, many of which have gender differences in growth rate. For example, female tilapias grow about 40% faster than males, and fish such as carp, flounder and half-smooth tongue sole, females grow faster than males. Individuals grow 30%-100% faster. Therefore, research on these fish sex-related genes and sex-specific molecular markers has important scientific significance, and has great application potential and promotion prospects. [0003] Cynoglossus semiliaevis (Cynoglossus semiliaevis) is a rare and valuable seawater fish endemic to my country. It belongs to warm-water demersal fish in coastal waters. It is distributed in coastal area...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈松林李静邓思平田永胜沙珍霞
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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