Method for predicting plant hybrid cultivation offspring ratio and application

Abstract

The present invention belongs to the field of new plant variety breeding method, and is especially breeding method of adopting anther cultivation technology in obtaining anther cultivation offspring lines of capsicum hybrid and predicting the type ratio of the anther cultivation offspring lines. The present invention performs anther cultivation of capsicum hybrid based on the characteristic of stable fruit shape character of plant anther cultivation offspring to obtain monoploid plant, doubles chromosome to obtain amphiploid lines, analyzes the fruit shapes of the lines to obtain fruit shape index values and regions of the prohybrid and the parents, and determines the ratio of the fruit shape index value regions. The ratio of fruit shape index similar to the prohybrid is maintained in 10-20 % and that to the parents in about 5 %, so as to obtain total anther cultivation line plant number meeting the breeding target.

Description

technical field [0001] The invention belongs to a method for breeding inbred lines of new plant varieties, and specifically relates to a breeding method for obtaining flower-cultured progeny lines of capsicum hybrids by using anther culture technology, and predicting the type ratio of flower-cultured progeny lines according to breeding needs . Background technique [0002] Sweet pepper anther haploid breeding technology is a set of biotechnology widely used in sweet pepper breeding research formed by the Beijing Haidian District Plant Tissue Culture Technology Laboratory for more than 20 years. The main point of this technology is to combine the hybrid The anthers are cultured in vitro (flower cultivation), and the pollen is induced to differentiate into haploid plants, and the diploid plants are formed after the chromosomes are artificially doubled to obtain its strain, that is, the DH strain, which is called "flower culture strain" in breeding. The genotype is homozygous ...

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Problems solved by technology

[0003] In our floriculture selection and breeding work, the inoculation materials determined are usually hybrids or double crosses with excellent comprehensive traits, especially their fruit traits, which meet our breeding goals within a certain period of time. Therefore, it is necessary to use the inoculation materials The anther culture mainly obtains the flower culture lines similar to the hybrid type or its parent type. Many years of breeding practice show that among the obtained flower culture lines, the separation of the fruit characters of each flower culture line is very obvious, and there are similar to the original hybrid. , there are similar to the parental type of the original hybrid, and there are also a certain amount of flower culture lines that are different from the original hybrid and its parents, and some flower culture lines that are useless are also produced. Sometimes when determining an inoculation material, Often due to various reasons, the flower culture lines that meet the breeding goals cannot be bred, resulting in a certain waste, which shows that there is a certain blindness in the breeding research.

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