Synergistic antifouling compositions comprising 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1h-pyrrole-3-carbonitrile
A technology of trifluoromethyl and chlorophenyl, applied in the field of material protection
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[0048] 1. Toxicant Petri Dish Test in 96-well Petri Dish
[0049] Activity against algal growth was determined by the poison plate assay. Activity against algal growth was determined by the poison plate assay. Calculated amounts of stock solutions (containing 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile, pitoxazine, DCOIT, difluoro Inhibitorin or to-afluzam) was pipetted into a multi-well petri dish to achieve a final test concentration ranging from 0.05ppm to 10ppm, and mixed with warm medium. The medium was inoculated with 300 microliters of algae suspension. The multiwell dishes were maintained at 21° C., 65% relative humidity and a 16 hour daily light-dark cycle (1000 Lux photoperiod).
[0050] The minimum inhibitory concentration (MIC) was taken as the lowest concentration of each test compound or mixture of test compounds sufficient to inhibit visible growth. Take the MIC as the end of activity. The endpoints of the combination of ingre...
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